| 三氧化二砷对肝癌细胞系HepG2生长及核基质蛋白的影响 |
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| 引用本文： | 于鼎,蔡志明,朱立元,王子慧,邱殷庆.三氧化二砷对肝癌细胞系HepG2生长及核基质蛋白的影响[J].华中医学杂志,2000,24(1):10-11,15,F004. |
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| 作者姓名： | 于鼎 蔡志明 朱立元 王子慧 邱殷庆 |
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| 作者单位： | 1. 深圳市中心医院外科深圳 518036
2. 香港中文大学医学院解剖系 |
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| 摘 要： | 目的 探讨三氧化二砷对人肝癌细胞系ＨｅｐＧ２的生长及核基质蛋白的影响。方法 应用生长指数分析和高分辨ＳＤＳ－聚丙烯酰胺交双向电泳比较用Ａｓ２Ｏ３处理后的ＨｅｐＧ２细胞主核基质蛋白的改变。结果 Ａｓ２Ｏ３抑制ＨｅｐＧ２的生长，双向电泳显示经Ａｓ２Ｏ３处理后ＨｅｐＧ２细胞内出现新的核基质蛋白，并且该蛋白是细胞特异性的。结论 核基质蛋白是某些抗癌药物的作用靶子，Ａｓ２Ｏ３抑帛 癌细胞的生长可望有用于临床
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| 关 键 词： | 三氧化三坤 HepG2 核基质蛋白 肝肿瘤 |
Effect of As2O3 on growth and nuclear matrix proteins of human hepatoblastoma cell line HepG2 |
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| Yu Ding,Wang Zihui,Cai Zhiming et al.Effect of As2O3 on growth and nuclear matrix proteins of human hepatoblastoma cell line HepG2[J].Central China Medical Journal,2000,24(1):10-11,15,F004. |
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| Authors: | Yu Ding Wang Zihui Cai Zhiming |
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| Affiliation: | Yu Ding,Wang Zihui,Cai Zhiming et al.Department of Surgery,ShenZhen Central Hospital,Shenzhen 518036 |
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| Abstract: | Objective To investigate the effect of arsenic trioxide (As 2O 3)on growth and nuclear matrix proteins of human hepatoblastoma cell line HepG2.Methods The growth rate of HepG2 treated with As 2O 3 was studied.By using high resolution two dimensional polyacrylamide gel electrophoresis,the nuclear matrix proteins of treated or untreated HepG2 were compared.Results The growth rate of HepG2 treated with As 2O 3 was reduced.2 D gel analysis showed the presence of novel nuclear matrix proteins in As 2O 3 treated tumor cells and these proteins are cell line specific.Conclusion As 2O 3 may reduce the growth of HepG2 cells.Nuclear matrix protein may be a target of As 2O 3. |
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| Keywords: | As 2O 3 HepG2 Nuclear matrix protein Two dimensional polyacrylamide gel electrophoresis |
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