ABCG2,ABCB5在皮肤黑素瘤中的共表达及意义

目的探讨ATP结合盒(ABC)转运体ABCG2,ABCB5在皮肤黑素瘤中的共表达及意义。方法采用免疫组化单/双重染色法检测30例皮肤黑素瘤、30例皮内痣及5例A375细胞移植瘤中ABCG2,AB-CB5的表达与共表达。结果①ABCG2在黑素瘤、皮内痣中阳性率分别为63.33%和0,两者相比差异有统计学意义(P<0.05),阳性细胞率平均值分别为(25.50±9.47)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(27.00±8.04)%;②ABCB5在黑素瘤、皮内痣中阳性率分别为73.33%和0,两者相比差异有统计学意义(P<0.05),阳性细胞率平均值分别为(28.75±2.22)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(30.50±4.66)%;③ABCG2和ABCB5在黑素瘤、皮内痣中共表达阳性率分别为60.00%和0,两者相比差异有统计学意义(P<0.05),共表达阳性细胞率平均值分别为(23.00±2.94)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(24.50±4.04)%。结论皮肤黑素瘤与A375细胞移植瘤中存在ABCG2,ABCB5的阳性表达和共表达,提示皮肤黑素瘤的多药耐药可能与ABCG2和ABCB5有关。

Co-expression of ABCG2 and ABCB5 in Malignant Melanoma

Objective To study co-expression of ABC transporter ABCG2 and ABCB5 in cutaneous malignant melanoma(CMM).Methods The expressions and co-expressions of ABCG2 and ABCB5 in 30 CMM,30 intradermal nevus and 5 transplanted tumors of A375 cells in nude mice were detected by immunohistochemical technique.Results ①The positive rates of ABCG2 expression in CMM and intradermal nevus were 63.33%and 0.There were significant statistically differences between them(P<0.05).The mean values of positive cell rates were (25.50±9.47)% and 0.There were significant differences between them.In A375 cells transplanted tumor the positive rates and the mean values of positive cell rates were 100.00%,(27.00±8.04)%.②The positive rates of ABCB5 expression in CMM and intradermal nevus were 73.33% and 0.There were significant statistically differences between them(P<0.05).The mean values of positive cell rates were (28.75±2.22)% and 0.There were significant differences between them.In A375 cells transplanted tumor the positive rates and the mean values of positive cell rates were 100.00%,(30.50±4.66)%.③The positive rates of ABCG2 and ABCB5 co-expression in CMM and intradermal nevus were 60.00%and 0.There was significant statistically differences between them(P<0.05).The mean values of positive cell rates were (23.00±2.94)% and 0.There were significant differences between them.In A375 cells transplanted tumor the positive rates and the mean values of positive cell rates were 100.00%,(24.50±4.04)%.Conclusions There were expressions and co-expressions of ABCG2 and ABCB5 in CMM and A375 cells transplanted tumor,these results imply that ABCG2 and ABCB5 maybe associated with the multidrug resistance in the chemical therapy of CMM.