卡巴胆碱对缺血再灌流大鼠肠黏膜组织炎症反应和血流量的影响

目的 研究缺血再灌流时卡巴胆碱对缺血再灌流大鼠肠组织炎症反应和血流量的影响.方法 Wistar大鼠开腹制做空肠袋,夹闭肠系膜上动脉(SMA)阻断血流45 min后恢复血流,制成肠缺血-再灌流模型.动物随机分为假手术组、缺血-再灌流+生理盐水组(I/R+NS)和缺血-再灌流+卡巴胆碱组(I/R+Ca).I/R+Ca组在SMA阻断血流同时向肠袋内注射卡巴胆碱(0.1mg/kg),I/R+NS组给予相同剂量的生理盐水.观察肠黏膜损伤情况;检测肠组织中DAO含量;ELLSA法测定肠组织中TNF-α含量;应用多普勒血流仪测定肠黏膜血流量.结果 I/R+Ca组与I/R+NS组相比,肠黏膜病理变化较轻.I/R+Ca组肠袋黏膜组织DAO活性较L/R+NS组显著增加(P<0.01);同时L/R+Ca组与I/R+NS组相比,肠黏膜血流量明显增加(P<0.01),而肠I/R+Ca组黏膜组织中TNF-α含量较I/R+Ns明显减少(P<0.01).结论 卡巴胆碱能促进缺血再灌流时肠黏膜血流恢复,增加肠黏膜血流量;抑制肠组织中TNF-α的生成,减轻肠黏膜病理损害,时肠黏膜具有保护作用.

Effects of carbachol on intestinal inflammation and mucosal blood flow in rats with gut ischemia-reperfusion

Objective To investigate the effects of carbachol on intestinal inflammation and mucosal blood flow after gut ischemia-repedusion(I/R) in rat. Method A jejunal sac was formed in Wistar rats. The superior mesenteric artery (SMA) was occluded for 45 mi-nutes followed by 240 minutes of reperfusion. Animals were random divided into three groups: sham operation, L/R + saline injection (I/R + NS) and I/R + carbachol injection (0.1mg/kg, I/R + Ca). Immediately after occluded of SAM blood flow, either 0.1mg/kg of carba-chol or same account of 0.9% saline was injected into the jejunal sac. The pathological injury was observed with HE staining. The activity of DAO and content of TNF-α in intestinal mucosa tissue were determined. Mucosal blood flow was measured by laser Doppler. All measure-ments were done at 0 min, 30 min, 60 min, 120 min, and 240 min after reperfusion. Result In I/R group the activity of DAO in intestinal mucosa and mucosal blood flow deceased, meanwhile the content of TNF-α gut tissue was dramatically increased than those in sham operation (P<0.01). Severe pathological changes were observed in intestinal mucosa. After injection of carbachol, the activity of DAO and mucosal blood flow increased (P<0.01), but the content of TNF-α in intestinal mucosa were dramatically decreased (P<0.01), compared with those in I/R group. Conclusion Administration of carbachol protects intestinal ischemia-reperfusion injury by attenuating intestinal mucosa inflammation and increasing gut mueosal blood flow.