Polymeric genes MEL8, MEL9 and MEL10 — new members of α-galactosidase gene family in Saccharomyces cerevisiae |
| |
| Authors: | Gennadi Naumov Elena Naumova Hilkka Turakainen Pirkko Suominen Matti Korhola |
| |
| Affiliation: | (1) All-Union Scientific Research Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi 1, 113545 Moscow, USSR;(2) Department of Genetics, University of Helsinki, Arkadiankatu 7, SF-00100 Helsinki, Finland;(3) Present address: Research Laboratories of the Finnish State Alcohol Company (Alko Ltd), P.O. Box 350, SF-00101 Helsinki, Finland |
| |
| Abstract: | Summary We used a combination of genetic hybridization analysis and electrokaryotyping with radioactively labelled MEL1 gene probe hybridization to isolate and identify seven polymeric genes for the fermentation of melibiose in strain CBS 5378 of Saccharomyces cerevisiae (syn. norbensis). Four of the MEL genes, i.e. MEL3, MEL4, MEL6 and MEL7, were allelic to those found in S. cerevisiae strain CBS 4411 (syn. S. oleaginosus) whereas three genes, i.e. MEL8, MEL9 and MEL10 occupied new loci. Electrokaryotyping showed that all seven MEL genes in CBS 5378 were located on different chromosomes. The new MEL8, MEL9 and MEL10 genes were found on chromosomes XV, X/XIV and XII, respectively. |
| |
| Keywords: | Saccharomyces cerevisiae Melibiose fermentation MEL Polymeric genes |
| 本文献已被 SpringerLink 等数据库收录！ |
|
