电压敏感染料di-4-ANEPPS的光谱研究

实验研究了电压敏感染料di-4-ANEPPS在家兔心肌组织中的吸收光谱和荧光光谱特性。结果表明,含染料组织的光吸收普遍大于对照组，在450～550 nm波段吸收谱差异更明显;染料在心室组织中的最大吸收峰为(479.75±0.44) nm。通过测量含染料心脏不同部位的荧光光谱，首次发现心室组织、心房组织和主动脉的最大荧光峰位有一定差异，其相对荧光强度则与染料的分布浓度有关。根据三维和二维荧光光谱分别确定了含染料心房和心室组织的最佳荧光激发波长和荧光测定波长。利用心房和心室组织的静息电位差，在不同波长激发光下测量了染料的荧光光谱移动，确定了光标测量实验的最佳激发光和相应荧光检测波长范围。这些研究结果为心脏光学标测系统的设计提供了理论依据。

Spectral Study of Voltage Sensitive Dye di-4-ANEPPS

This study investigated the absorption spectrum and the fluorescence spectrum of rabbit hearts stained with the voltage sensitive dye (di-4-ANEPPS). The results suggested that the optical absorption of tissue with the dye was higher than that of the control group, and there were significant differences between the experimental group and control group in the range of 450-550 nm. It was also indicated that the maximum absorption peak of the dye in tissues was at (479.25 +/- 0.44) nm. Additionally, the different peaks of fluorescence emission spectra from the atriums, ventricles and aorta were originally found by testing the five parts of rabbit hearts with the dye. Their relative intensities were related to the distribution concentrations of the dye. Meanwhile, the peaks of excitation spectra and fluorescence emission spectra were determined by examining the three-dimensional and two-dimensional fluorescence spectra using ventricles and atriums with the dye. Based on the discrepancy of rest membrane voltages between ventricles and atriums, the best wavelength ranges of excitation light and emissions light of optical mapping were determined by the shifts of the dye in emission spectra with excitation at different wavelengths. These results offer a theoretical foundation for the design of a cardiac optical mapping system.