菜心种质材料的分子身份证构建研究

建立菜心种质材料的分子身份证有利于菜心种质材料的收集、保存、管理和利用.利用荧光MFLP标记技术分析供试的36份菜心种质材料,从200对选择性扩增引物组合中筛选出扩增产物清晰稳定的18对引物组合.这些引物组合在36份菜心种质材料中有3～31等位变异,多态性信息量(PIC)和标记指数值(MI)分别在0.26～0.98和0.8～30.4之间.主坐标分析表明,18对引物组合可以区分各供试材料,并按遗传相似性将供试材料归为3个类群.考虑到使用最少引物组合区分不同种质材料,筛选和确定出PIC和MI值最高的3个MFLP选择性扩增引物组合(tfCCTA(CA)7/mAAG、tfGGTC(ATT)5/mCAA和tfCCTA(CA)7/mCTC)作为核心引物组合,建立3个引物组合检测到每份种质材料等位变异有(1)无(0)的二进制数据,并将其转换成简捷直观的16进制数据,从而构建了供试菜心种质材料的分子身份证.

Establishment of molecular identity in flowering Chinese cabbage germplasm

Eestablishment of molecular identity is beneficial to effectively serve the preservation and utilization of the germplasm in flowering Chinese cabbage. In this study, the fluorescent microsatellite -anchored fragment length polymorphism (MFLP) technique was utilized to genotype 36 accessions of the flowering Chinese cabbage germplasm collected from different parts of China, and 18 appropriate primer pairs amplified stably and unambiguously polymorphic bands were chosen from 200 selective amplification primer combinations to detect the polymorphic alleles among the accessions. The results showed that the polymorphic alleles for 18 primer pairs ranged from 3 to 31 with the average of 12.5 per primer combination. Polymorphism information content (PIC) and marker index (MI) ranged in 0.26-0.98 and 0.8- 30.4, respectively. The principal coordinate analysis (PCA) showed that 36 accessions could be clearly distinguished by 18 primer combinations, which divided into 3 groups based on genetic similarity. According to the principal of distinguishing accessions with the less primer pairs, three primer pairs including tfCCTA(CA)7/mAAG, tfGGTC(ATT)5/mCAA and tfCCTA (CA)7/mCTC with the highest values of PIC and MI were selected as the core primer combinations. Based on the binary data of the allele variants (allele present is 1, absent is 0) of the accessions, the molecular identity for each accession of flowering Chinese cabbage was established by converting the corresponding binary data into the simple and intuitive hexadecimal data.