远志总皂苷大孔吸附树脂纯化工艺研究

目的:优选远志总皂苷大孔树脂纯化工艺。方法:在以细叶远志皂苷的纯度为指标,考察多种型号大孔树脂纯化远志总皂苷的吸附及洗脱条件。结果:HPD-100型大孔吸附树脂为最佳纯化远志总皂苷的大孔树脂。最佳纯化工艺为上样液细叶远志皂苷的质量浓度为0.5 g/ml,以蒸馏水3 BV、30%乙醇、70%乙醇各4 BV以1 BV/h速度依次洗脱,收集70%乙醇洗脱部位。结论:该法工艺可较好地纯化远志总皂苷且稳定可行,为新药研发提供了一定的依据。

Purification Technology of Saponins from Radix Polygalae by Macroporous Adsorbtion Resin

Objective：To optimize the purification technology of Saponins from Radix Polygalae with macroporous adsorbtion resins. Methods：The absorption and elution conditions of Saponins from Radix Polygalae purificatin by various maeroporous adsorption resins were investigated with the purification of tenuifolin as index. Results HPD - 100 type maeroporous adsorption resin was the best choice for the purification of Saponins from Radix Polygalae. The optimal concentration of ethanol solution was 0.5 g/ mL. And the optimal elution condition was determined as follows：water （3 BV） ,30% ethanol （4 BV） ,70% ethanol （4BV） in sequence,volume flow at 1 BV/h. And the elution parts of 70% enthol were collected. Conclusion：The process in the research could significantly increase the purity of Saponins from Radix Polygalae and lay the foundation for further research.