| 海洋细菌NBRC10260产琼胶酶发酵条件优化 |
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| 引用本文： | 朱启忠,朱慧文,孙艳娜,周晓龙,郝梅,郭振博,施超. 海洋细菌NBRC10260产琼胶酶发酵条件优化[J]. 中国酿造, 2011, 0(5) |
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| 作者姓名： | 朱启忠 朱慧文 孙艳娜 周晓龙 郝梅 郭振博 施超 |
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| 作者单位： | 山东大学,海洋学院,山东,威海,264200 |
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| 基金项目： | 国家创新性基金，山东大学科技立项重点项目专项基金 |
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| 摘 要： | 通过正交试验法对产琼胶酶海洋菌株NBRC102603发酵条件进行优化,优化培养基为:蛋白胨4.0g/L、酵母粉1.25g/L、琼胶粉5.0g/L;在装液量150mL(500mL三角瓶)、28℃、150r/min、接种量为1%条件下发酵48h酶活力达到最高,为56.89U/mL,比未优化前提高了2.01倍.
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| 关 键 词： | 海洋细菌 琼胶酶 正交试验 |
Optimization of fermentation conditions for agarase production by a marine bacterium NBRC10260 |
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| ZHU Qizhong,ZHU Huiwen,SUN Yanna,ZHOU Xiaolong,HAO Mei,GUO Zhenbo,SHI Chao. Optimization of fermentation conditions for agarase production by a marine bacterium NBRC10260[J]. China Brewing, 2011, 0(5) |
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| Authors: | ZHU Qizhong ZHU Huiwen SUN Yanna ZHOU Xiaolong HAO Mei GUO Zhenbo SHI Chao |
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| Affiliation: | ZHU Qizhong,ZHU Huiwen,SUN Yanna,ZHOU Xiaolong,HAO Mei,GUO Zhenbo,SHI Chao (Marine College,Shandong University at Weihai,Weihai 264200,China) |
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| Abstract: | The fermentation conditions of an agarase-producing marine bacterium NBRC10260 were optimized by orthogonal test.The optimal culture medium consisted of 4.0g/L peptone,1.25g/L yeast extract and 5.0g/L agar.The highest agarase activity(56.89U/ml) was obtained when the fermentation was conducted in 500ml Erlenmeyer flask containing 150ml medium with shaking rate of 150r/min and inoculum of 1% for 48h.The agarase activity was increased 3.01 times after optimization. |
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| Keywords: | marine bacterium agarase orthogonal test |
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