| 羟自由基(OH)、超氧阴离子(O2-)与线粒体低水平化学发光相关性的研究 |
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| 引用本文： | 马玉琴,赵克俭,张仲纶,苏震,马斌,郑雁珍,杨光洁,米志勇,陈昕.羟自由基(OH)、超氧阴离子(O2-)与线粒体低水平化学发光相关性的研究[J].影像科学与光化学,1994,12(4):312-317. |
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| 作者姓名： | 马玉琴 赵克俭 张仲纶 苏震 马斌 郑雁珍 杨光洁 米志勇 陈昕 |
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| 作者单位： | 中国科学院生物物理研究所, 北京100101 |
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| 摘 要： | 以线粒体为模式体系,研究了低水平化学发光的过程和机制。实验发现在一定浓度范围(蛋白0-100毫克/毫升)内,线粒体悬液的发光是随浓度的升高而增强。最适宜的测量条件为35℃和pH=7.A.注入外源性·OH和·OH的清除剂证实,OH可起动脂质过氧化反应,且1O2是构成发光的体内光子源。超氧化物歧化酶(SOD)对线粒体发光有调节作用。
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| 关 键 词： | 线粒体 低水平化学发光 活性氧 |
| 收稿时间： | 1994-01-12 |
STUDY ON CORRELATION BETWEEN ·OH,O2·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA |
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| MA YU-QIN,ZHAO KE-JIAN,ZHANG ZHONG-LUN,SU ZHEN,MA BIN,ZHENG YAN-ZHEN,YANG GUANG-JIE,MI ZHI-YONG,CHEN XIN.STUDY ON CORRELATION BETWEEN ·OH,O2·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA[J].Imaging Science and Photochemistry,1994,12(4):312-317. |
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| Authors: | MA YU-QIN ZHAO KE-JIAN ZHANG ZHONG-LUN SU ZHEN MA BIN ZHENG YAN-ZHEN YANG GUANG-JIE MI ZHI-YONG CHEN XIN |
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| Affiliation: | Institute of Biophysics, Academia Sinica, Beijing 100101, P. R. China |
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| Abstract: | Mitochondria was used a sa model system to study the course and mechanism of low-level chemiluminescence.It is discovered that the LCL of mitochondria suspension riseswith its concentration increasing under the definited range (0-100 mg/mL protein).Thebest satisfactory condition is 35℃ and PH=7.4.By adding of exogenous ·OH and ·OH cleaner, it is convicted that ·OH can initiate lipld peroxidation reactions and 1O2 is one of the main photon sources.SOD(in vivo) may adjust the LCL from mitochondria. |
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| Keywords: | mitochondria low-level chemiluminescence activated oxygen |
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