Connexin 43和E-cadherin在非小细胞肺癌中表达及相关性研究

背景与目的由连接蛋白(connexin，Cx)构成的细胞间隙连接(gapjunmion)对细胞的增殖和分化起着重要的调控作用，Cx表达下降将导致细胞恶化。上皮钙粘蛋白(E-cadherin)为上皮细胞与细胞之间及细咆与细胞外基质粘附的跨膜糖蛋白分子，其功能抑制或表达下降可使细胞间粘附能力下降，细胞易于分离。本研究旨在探讨连接蛋白43(Connexin 43，Cx43)和E—cadherin在非小细胞肺癌中的表达及两者的相互关系。方法采用免疫组织化学S—P法检测85例原发性非小细胞肺癌组织的Cx43和E—cadherin蛋白表达，并进行两者的相关性分析。结果Cx43和E—cadherin蛋白在非小细胞肺癌中表达显著下降．其下降程度与肺癌的细胞分化程度、pTNM分期和有无淋巴结转移均有密切关系，而与组织学分型无明显关系。Cx43和E—cadherin蛋白表达之间存在明显的相关性。结论Cx43和E—cadherin蛋白在非小细胞肺癌中表达显著下降，且两者存在着明显的相关性，可能是肺癌发生和发展过程中的共同事件。

Expressions of connexin 43 and E-cadherin and their correlation in non-small cell lung cancer

Background and objective Connexin (Cx), a transmembranous protein, makes up of gap junction, which induces the communication of cells and plays an important role in proliferation and differentiation of cells. The directly or indirectly reduced expression of connexin protein may induce a malignant tendency of cells. E-cadherin is a transmembranous glycoprotein and induces the adhesion of epitheliums and extracellular matrix. The reduced expression or dysfunction of E-cadherin will impair the ability of adhesion and make cells easy to be isolated. The aim of this study is to examine the expressions of Connexin 43 (Cx43) and E-cadherin in non-small cell lung cancer (NSCLC), and to analyze their correlation. Methods The expressions of Cx43 and E-cadherin proteins were detected in 85 primary NSCLC samples by immunohistochemistry S-P method, and their correlation was then analyzed. Results The expressions of Cx43 and E-cadherin were remarkably decreased in NSCLC tissues. Cx43 and E-cadherin expressions were related to cell differentiation, pTNM stage and lymphatic metastasis of NSCLC. The expression of Cx43 significantly correlated with the expression of E-cadherin. Conclusion The expressions of Cx43 and E-cadherin significantly decrease in NSCLC and they correlate with each other. It might be common affairs in carcinogenesis and development of NSCLC.