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磷脂脂肪酸分析在湖泊沉积物微生物生态学研究中的应用
引用本文:赵大勇,燕文明,冯景伟,袁守军.磷脂脂肪酸分析在湖泊沉积物微生物生态学研究中的应用[J].化学与生物工程,2009,26(12):17-20.
作者姓名:赵大勇  燕文明  冯景伟  袁守军
作者单位:1. 河海大学水文水资源学院,江苏,南京,210098
2. 河海大学水文水资源与水利工程科学国家重点实验室,江苏,南京,210098
3. 合肥工业大学土木与水利工程学院,安徽,合肥,230009
基金项目:河海大学引进人才科研启动经费资助项目,河海大学国家重点实验室团队创新基金子课题 
摘    要:磷脂脂肪酸分析(PLFA)技术是近年来出现的一种新的微生物生态学研究方法,它是利用微生物细胞内磷脂脂肪酸的成分和含量相对稳定的原理,通过对环境样品中的磷脂脂肪酸进行提取和分析,以获得微生物生物量和群落结构变化的信息,已被应用于土壤、水体和植物根系等环境中微生物生态学的研究.综述了PLFA技术在湖泊沉积物微生物生态学研究中的应用,同时指出了PLFA技术存在的问题及其发展前景.

关 键 词:磷脂脂肪酸分析  湖泊沉积物  微生物生态学

Applications of Phospholipid Fatty Acid Analysis in the Microbial Ecology Studies of Lake Sediment
ZHAO Da-yong,YAN Wen-ming,FENG Jing-wei,YUAN Shou-jun.Applications of Phospholipid Fatty Acid Analysis in the Microbial Ecology Studies of Lake Sediment[J].Chemistry & Bioengineering,2009,26(12):17-20.
Authors:ZHAO Da-yong  YAN Wen-ming  FENG Jing-wei  YUAN Shou-jun
Affiliation:ZHAO Da-yong1,YAN Wen-ming2,FENG Jing-wei3,YUAN Shou-jun3 (1.College of Hydrology and Water Resources,Hohai University,Nanjing 210098,China,2.State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,3.School of Civil and Hydraulic Engineering,Hefei University of Technology,Hefei 230009,China)
Abstract:Phospholipid fatty acid analysis(PLFA) is a recently developed technology for the study of microbial ecology.The principle of PLFA is the compositions and contents of phospholipid fatty acids in cells are relatively stable.The information about the biomass and microbial community structure could be acquired through extraction and analysis the phospholipid fatty acid in environmental samples.PLFA has been widely used in the microbial ecology studies of soil,water and plant root.This paper reviews application...
Keywords:phospholipid fatty acid analysis  lake sediment  microbial ecology  
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