脂多糖刺激RAW264.7和Ana-1形态改变及细胞因子表达的差异 |
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引用本文: | 石榴,方怡,袁伟锋,李理,黄文杰. 脂多糖刺激RAW264.7和Ana-1形态改变及细胞因子表达的差异[J]. 国际呼吸杂志, 2004, 29(1): 772-776. DOI: 10.3760/cma.j.issn.1673-436X.2009.013.002 |
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作者姓名: | 石榴 方怡 袁伟锋 李理 黄文杰 |
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作者单位: | 广州军区广州总医院呼吸内科,510010; |
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摘 要: | Objective To compare the morphologic change and cytokines expression in RAW264.7 and Ana-1 stimulated by lipopolysaccharide(LPS). Methods RAW264.7 and Ana-1 were cultivated with various concentrations of LPS(0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L). MTT was performed to evaluate the proliferation ability of cells. Two kinds of cells were cultivated with 1 mg/L. Then,the concentrations of TNF-α,IL-1β,IL-6,IL-10 in culture medium were detected by ELISA in different times(0 h,4 h,8 h,12 h,24 h). Results The survival rates of RAW264.7 and Ana-1 (162.05±28.14)% and (159.92±20.43)% were significantly higher in 1 mg/L group than those of other groups. When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 expressed cytokines in time-dependent manner (increased first and decreased finally). The concentrations of TNF-α and IL-10 in RAW264.7 group were higher than those Ana-1 group in 4 h only. IL-1β and IL-6,however,were in higher concentrations in RAW264.7 group than Ana-1 group all the time. Conclusions When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 have higher survival rate. RAW264.7 and Ana-1 have different ability in expressing cytokines after being stimulated by LPS. Therefore,RAW264.7 and Ana-1 have variant response to LPS slightly.
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关 键 词: | 脂多糖 小鼠巨噬细胞 细胞因子 |
Comparison between two kinds of murine macrophages.Ana-1 and RAW264.7 cell in appearance and cytokines expression |
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Keywords: | RAW264.7Ana-1LipopolysaccharideMurine macrophageRAW264.7 eellAna-1 cellCytokine |
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