| 藤黄酸联合顺铂对人骨肉瘤细胞作用的实验研究 |
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| 引用本文： | 巴成磊,关国发,由长城,赵伟,朱凯.藤黄酸联合顺铂对人骨肉瘤细胞作用的实验研究[J].中国矫形外科杂志,2011,19(23):2000-2002. |
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| 作者姓名： | 巴成磊 关国发 由长城 赵伟 朱凯 |
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| 作者单位： | 哈尔滨医科大学附属第一医院骨科;哈尔滨儿童医院影像科; |
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| 摘 要： | 目的]本实验主要研究藤黄酸(gambogic acid,GA)在骨肉瘤中的抗肿瘤作用,检验藤黄酸与传统的骨肉瘤化疗药物顺铂(cisplatin,CDDP)联用是否具有协同作用,探讨其作用的机制。方法]采用CCK-8法测定不同浓度藤黄酸、顺铂单用及联合应用对人骨肉瘤细胞MG-63的增殖抑制作用,PI染色流式细胞术检测细胞周期变化,Annexin V-FITC/PI双染色检测细胞凋亡变化。结果]藤黄酸对MG-63具有明显生长抑制作用,且具有浓度依赖性,IC 25为0.52μg/ml,藤黄酸诱导MG-63细胞产生G2/M期细胞周期阻滞和凋亡,而顺铂则诱导产生G0/G1期细胞周期阻滞和凋亡。藤黄酸与顺铂联用时增殖抑制及诱导凋亡作用比单用时明显增强。结论]藤黄酸通过诱导MG-63细胞周期阻滞和凋亡产生抗人骨肉瘤细胞增殖作用,藤黄酸与顺铂联用具有协同作用。
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| 关 键 词： | 骨肉瘤 藤黄酸 细胞周期 凋亡 |
Experimental study on effects of gambogic acid with cisplatin to human osteosarcoma cells |
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| BA Cheng-lei,GUAN Guo-fa,YOU Chang-cheng,et al..Experimental study on effects of gambogic acid with cisplatin to human osteosarcoma cells[J].The Orthopedic Journal of China,2011,19(23):2000-2002. |
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| Authors: | BA Cheng-lei GUAN Guo-fa YOU Chang-cheng |
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| Affiliation: | BA Cheng-lei,GUAN Guo-fa,YOU Chang-cheng,et al.Department of Orthopedics,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China |
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| Abstract: | Objective]To investigate whether there is a synergistic effect of antitumor activity of gambogic acid(GA) with cisplatin(CDDP),and its mechanisms are also investigated. Methods]GA alone or combined with CDDP was used to treat human ostoesarcoma cells line MG-63 by CCK-8 assay.Cell cycle distribution was detected by PI staining and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITC and PI. Results]GA had an significant growth inhibition on MG-63 in a concentration-dependent man... |
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| Keywords: | osteosarcoma gambogic acid cell cycle apoptosis |
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