山东长清栝楼及丹参根结线虫病原虫种类研究

分离鉴定危害栝楼和丹参的植物根结线虫，为两种药用植物根结线虫的防治提供理论依据。采用贝曼漏斗法自采集来的丹参和栝楼根中分离根结线虫，分离后采用光学显微镜观察其形态。结果发现了6种植物根结线虫，其中丹参中分离到5种，即咖啡短体线虫（Pratylenchus coffeae）、南方根结线虫（Meloidogyne incognita）、穿刺短体线虫（Pratylenchus penetrans）、美洲剑线虫（Xiphinema americanum）、纤毛棘刺线虫（Helicotylenchus apiculus）。栝楼中分离到2种，即美洲剑线虫（Xiphinema americanum）、假强壮螺旋线虫（Helicotylenchus pseudorobustus）。该研究可为山东长清地区栝楼及丹参根部病害的防治提供参考依据。

Research on the species of root-knot nematode pathogens on Trichosanthes kirilowii Maxim.and Salvia miltiorrhiza Bunge in Changqing,Shandong Province

In this paper, plant root-knot nematodes harmful to the Trichosanthes kirilowii Maxim. and Salvia miltiorrhiza Bunge were isolated and identified, providing a theoretical basis for the control of root-knot nematodes in two medicinal plants. Root-knot nematodes were isolated from the roots of Salvia miltiorrhiza and Trichosanthes kirilowii Maxim. collected by the Baermann funnel method. After isolation, the morphology of root-knot nematodes was observed by optical microscope. The results showed that six species of plant root parasitic nematodes were found. Pratylenchus coffeae, Meloidogyne incognita, Pratylenchus penetrans, Xiphinema americanum, Helicotylenchus apiculus. were isolated from Salvia miltiorrhiza, while Xiphinema americanum, Helicotylenchus pseudorobustus were isolated from Trichosanthes kirilowii. This experiment can provide some reference for the prevention and treatment of root diseases of Trichosanthes kirilowii and Salvia miltiorrhiza in Changqing area of Shandong province.