| 天麻中天麻素标准物质的研制 |
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| 引用本文： | 初侨,席兴军,董跟来,赵新颖,王尉,王风贺,张维冰,兰韬.天麻中天麻素标准物质的研制[J].山东科学,2019,32(1):21-28. |
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| 作者姓名： | 初侨 席兴军 董跟来 赵新颖 王尉 王风贺 张维冰 兰韬 |
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| 作者单位： | 1.中国标准化研究院农业与食品标准化研究所，北京 100191；2. 亚东信基（北京）农产品有限公司，北京 100125； 3.北京市理化分析测试中心，北京 100089；4. 南京师范大学环境学院，江苏 南京 210023； 5. 华东理工大学化学与分子工程学院，上海 200237 |
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| 基金项目： | 国家重点研发计划（2017YFF0211000）；国家质量监督检验检疫总局科技计划（2017QK108）;中央级公益性科研院所基本科研业务费（562017Y-5284） |
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| 摘 要： | 以天麻提取液为基本原料，建立了完备的高纯度天麻素高效制备液相色谱方法，制备了天麻素标准物质，并以紫外、红外、质谱、核磁共振波谱对其进行结构表征。以高效液相色谱法结合面积归一化法对该标准物质进行了均匀性、稳定性检验和8家实验室联合定值。结果表明，该标准物质均匀性较好，同时在0~4 ℃储存条件下2年内稳定性良好，定值结果显示其纯度标准值达到99.7%，置信水平为95%时的扩展不确定度为0.6%（k=2）。该标准物质达到了国内标准物质研制的技术要求，可为天麻及其相关产品分析检测结果的准确性、可比性和溯源性提供保证。
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| 关 键 词： | 天麻素 标准物质 均匀性 稳定性 不确定度 |
| 收稿时间： | 2018-09-21 |
Development of gastrodin certified reference material from the extract of Gastrodia elata Bl. |
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| CHU Qiao,XI Xing-jun,DONG Gen-lai,ZHAO Xin-ying,WANG wei,WANG Feng-he,ZHANG Wei-bing,LAN Tao.Development of gastrodin certified reference material from the extract of Gastrodia elata Bl.[J].Shandong Science,2019,32(1):21-28. |
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| Authors: | CHU Qiao XI Xing-jun DONG Gen-lai ZHAO Xin-ying WANG wei WANG Feng-he ZHANG Wei-bing LAN Tao |
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| Affiliation: | 1. Sub-Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing 100191, China；2. Yadongxinji (Beijing) Agricultural Products Co., Ltd.; Beijing 100022, China；3. Beijing Center for Physical and Chemical Analysis, Beijing 100089, China；4.School of Environment, Nanjing Normal University, Nanjing 210023, China；5.School of chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China |
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| Abstract: | In this paper we discussed the development method of gastrodin reference material from the extract of Gastrodia elata. The qualitative analysis of gastrodin was identified by UV, IR, MS and NMR spectrum. The inspection of uniformity, stability and value measurement of 8 qualified laboratories was carried out by HPLC combined with area normalization. The results showed the uniformity of this reference material is good. The material is stable in 2 years under 0~4 ℃ storage conditions. The purity of this material is 99.7% with the expanded uncertainty of 0.6% (k=2) in the confidence interval of 95%. The reference material meets the technical requirements for the development of domestic reference materials. It can provide assurance for the accuracy, comparability and traceability of the analytical results of Gastrodia elata and its related products. |
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| Keywords: | gastrodin certified reference material uniformity stability uncertainty |
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