| 哮喘患儿外周血Th1/Th2、Tc1/Tc2细胞检测及其意义 |
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| 引用本文： | 檀卫平,麦友刚,麦贤弟,李晓圆,吴葆菁,黄花荣,李静,朱昌国,黄绍良. 哮喘患儿外周血Th1/Th2、Tc1/Tc2细胞检测及其意义[J]. 实用医学杂志, 2005, 21(19): 2138-2140 |
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| 作者姓名： | 檀卫平 麦友刚 麦贤弟 李晓圆 吴葆菁 黄花荣 李静 朱昌国 黄绍良 |
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| 作者单位： | 510120,广州市,中山大学孙逸仙纪念医院儿科 |
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| 摘 要： | 目的:探讨哮喘患儿急性发作期及缓解期Th1/Th2、Tc1/Tc2细胞亚群平衡的变化情况。方法:采用荧光标记抗体,应用三色荧光流式细胞仪技术,从单细胞水平对急性发作期哮喘患儿(20例)、缓解期哮喘患儿(18例)及同龄正常健康儿(对照组20例)外周血CD4+、CD8+细胞内分泌IFN-γ、IL-4进行检测。结果:哮喘患儿外周血细胞内分泌IL-4(+)的CD4+(Th2)、CD8+(Tc2)百分率较正常对照组升高,且哮喘急性发作期高于缓解期;分泌IFN-γ(+)的CD4+(Th1)、CD8+(Tc1)百分率较正常对照组降低,且哮喘急性发作期低于缓解期。结论:哮喘患儿外周血Th2、Tc2百分率升高,Th1、Tc1百分率降低,Th1/Th2、Tc1/Tc2比值降低,且哮喘急性发作期低于缓解期。
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| 关 键 词： | 哮喘 Th1细胞 Th2细胞 T淋巴细胞 细胞毒性 |
| 收稿时间： | 2005-03-01 |
| 修稿时间： | 2005-03-01 |
Detection and clinical significance of peripheral Th1/Th2 and Tc1/Tc2 in asthmatic children |
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| TAN Wei-ping,MAI You-gang,MAI Xian-di,LI Xiao-yuan,WU Bao-jing,HUANG Hua-rong,LI Jing,ZHU Chang-guo,HUANG Shao-liang. Detection and clinical significance of peripheral Th1/Th2 and Tc1/Tc2 in asthmatic children[J]. The Journal of Practical Medicine, 2005, 21(19): 2138-2140 |
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| Authors: | TAN Wei-ping MAI You-gang MAI Xian-di LI Xiao-yuan WU Bao-jing HUANG Hua-rong LI Jing ZHU Chang-guo HUANG Shao-liang |
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| Affiliation: | Department of Pediatric, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China |
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| Abstract: | Objective To investigate the altering of Th1/Th2 and Tc1/Tc2 pattern in asthmatic children. Methods The intracellular secretion of IFN- γ and IL- 4 in peripheral CD4+ and CD8+ T lymphocytes of asthmatic children(n=38)and control group(n=20) were determined by FACS through using corresponding fluorescent antibodies.Results The percentage of intracellular IL- 4(+ )secretion CD4+ ( Th2) and CD8+ ( Tc2) in peripheral T lymphocytes of asthmatic children was higher than that of control group(P< 0.05) and higher i... |
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| Keywords: | Asthma Thl ceils Th2 cells T-lymphocytes cytotoxic |
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