不同接种污泥的厌氧氨氧化反应器启动特性及菌群结构演替规律分析

采用两套相同的膨胀颗粒污泥床(EGSB),分别接种城市污水处理厂活性污泥(A)和厌氧颗粒污泥(B)启动厌氧氨氧化反应器,考察了A、B反应器中污泥的形态、脱氮性能、容积氮负荷和氮去除负荷的差异,同时利用高通量测序技术从分子生物学水平分析启动过程中菌群结构演替规律.结果表明:反应器运行119 d后,A、B反应器均成功培养出成熟的厌氧氨氧化颗粒污泥,其中相比于B反应器,A反应器缩短了厌氧氨氧化启动过程的菌体自溶和活性迟滞阶段.微生物群落结构分析结果进一步表明,在活性迟滞和活性提高阶段,A反应器中浮霉菌门(Planctomycetes)的丰度分别为1.18%、5.98%,而B颗粒污泥反应器中浮霉菌门(Planctomycetes)的丰度仅为0.92%、1.41%,说明A反应器Planctomycetes菌在菌体自溶和活性迟滞阶的增长速度大于B反应器.此外,经过119 d的启动,A反应器中Candidatus Brocadia丰度可以达到11.34%,而B反应器中Candidatus Brocadia丰度仅为7.28%.

Start-up of ANAMMOX enrichment with different inoculated sludge and analysis of microbial community structure shift

Two anoxic EGSB reactors were compared for anaerobic ammonium oxidation (ANAMMOX) enrichment with different inoculated sludge, the activated sludge of urban sewage treatment plant (A) and anaerobic granular sludge (B). in this study, the performance of reactor were investigated from different aspects, including the morphologies of sludge, the performance of nitrogen removal, the nitrogen loading rate and the nitrogen removal rate. Meanwhile, Illumina Miseq sequencing was conducted to analyze the microbial community dynamics from the molecular level. The results show that the anammox granular sludge were both formed in A and B reactors after 119 days of cultivation. Compared with reactor B, The cell lysis and lag phases were shortened in reactor A. The Planctomycetes in A reactor were grown faster than in B reactor, and its abundance reached respectively 1.18% and 5.98% in A reactor at cell lysis and lag phases, which was higher than 0.92% and 1.41% in B bioreactor. Candidatus Brocadia were the dominated genera and its abundance reached 11.34% and 7.28% in reactor A and B.