Tamoxifen-loaded solid lipid nanoparticles-induced apoptosis in breast cancer cell lines |
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Authors: | Roghayeh Abbasalipourkabir Aref Salehzadeh Rasedee Abdullah |
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Affiliation: | 1. Department of Biochemistry, Hamadan University of Medical Sciences, Hamadan 6517619651, Iran;2. Department of Entomology and Toxicology, Hamadan University of Medical Sciences, Hamadan 6517619651, Iran;3. Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
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Abstract: | An in vitro study was conducted to determine the apoptosis induced by tamoxifen (TAM) and TAM-loaded solid lipid nanoparticles (SLNs) in breast cancer cell lines, MCF-7 and MDA-MB231 cells. The effect of free drug and drug-loaded SLN on the cell lines was characterised by cell morphology and cell cycle distribution using phase contrast microscopy, nuclear morphology and flow cytometry, respectively. The results showed that TAM-loaded SLNs have an equally efficient cytotoxic activity against MCF-7 and MDA-MB231 cells, compared to free TAM, and the half maximal inhibitory concentration (IC50) of TAM-loaded SLNs was generally lower than that of free TAM. In the presence of TAM and TAM-loaded SLN, the viability of the both cells diminishes and the cancer cells lose their normal morphological characteristics, detaches, aggregates and later develops apoptotic bodies. Flow cytometry analysis showed that TAM-loaded SLN like the free TAM caused a dose- and time-dependent apoptosis without cell cycle arrest of human breast cancer cells. Therefore, TAM-loaded SLN has great potential in human medicine for the treatment of breast cancers. |
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Keywords: | apoptosis breast cancer cell lines solid lipid nanoparticles tamoxifen |
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