| 利用大孔树脂纯化荷叶黄酮动态吸附条件的研究 |
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| 引用本文： | 陈佳佳,王在贵,刘朝良,张璐,方龙发. 利用大孔树脂纯化荷叶黄酮动态吸附条件的研究[J]. 中国饲料, 2011, 0(17) |
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| 作者姓名： | 陈佳佳 王在贵 刘朝良 张璐 方龙发 |
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| 作者单位： | 安徽农业大学生命科学学院 |
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| 基金项目： | 动物营养学国家重点实验室开放基金项目(2004DA125184F1104); 安徽农业大学稳定和引进人才基金项目 |
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| 摘 要： | 本试验从荷叶中提取黄酮类化合物,通过大孔树脂对黄酮的静态吸附效果的比较,研究X-5、HP-20、AB-8及HPD-100四种大孔吸附树脂的吸附效果,然后使用通过动态吸附与解吸试验就上样流速、上样液浓度、pH、解吸曲线以及洗脱剂的浓度对大孔吸附树脂(HP-20)的吸附效果进行研究,确定荷叶黄酮的分离纯化的最佳技术参数。结果表明,HP-20分离效果最好,静态饱和吸附量可达45.84 mg,解吸量为41.20 mg,解吸率为89.9%;在提取液总黄酮含量为0.50～0.70 mg/mL,pH为提取原液4.6左右,上样流速为1.5 mL/min的条件下,HP-20对荷叶总黄酮的吸附量可以达到5.20 mg/mL,体积分数越高的乙醇水溶液洗脱效果越好。
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| 关 键 词： | 荷叶黄酮 大孔吸附树脂 纯化 |
Extraction of flavonoids from lotus leaf by resins |
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| Chen Jiajia,Wang Zaigui,Liu Chaoliang,et al.. Extraction of flavonoids from lotus leaf by resins[J]. China Feed, 2011, 0(17) |
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| Authors: | Chen Jiajia Wang Zaigui Liu Chaoliang et al. |
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| Affiliation: | Chen Jiajia,Wang Zaigui,Liu Chaoliang,et al. |
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| Abstract: | This experiment compared the absorption effect of 4 kinds of macroporous resins X-5,HP-20,AB-8 and HPD-100.Then the effect of flow rate,sample density,on-like pH,desorption curves as well as the concentration of eluent on absorption of macroreticularresin(HP-20) was determined by dynamic testing of absorption and desorption to get the best technical parameters.The results showed that HP-20 was the best one.Its static absorption could reach 45.84 mg and desorption to 41.20 mg.It's desorption rate was much hi... |
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| Keywords: | lotus flavones resin purification |
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