星形细胞瘤p16与Rb蛋白的表达及其相关性研究

目的检测ｐ１６与Ｒｂ蛋白在原发性星形细胞瘤中的存在状况，以探讨不同病理类型星形细胞瘤中ｐ１６与Ｒｂ蛋白表达及其相关性。方法使用抗ｐ１６及Ｒｂ蛋白抗体对１０２例星形细胞瘤手术标本进行免疫细胞化学检测。结果低度恶性星形细胞瘤（ＷＨＯⅠ～Ⅱ级）中ｐ１６及Ｒｂ蛋白均为阳性表达，在高度恶性星形细胞瘤（ＷＨＯⅢ～Ⅳ级）中其阳性表达分别为４８．１％（２６／５４）和５７．４％（３１／５４）。在３１例Ｒｂ蛋白阳性标本中，有２４例（７７．４％）显示ｐ１６蛋白表达缺失或低表达，而在２３例Ｒｂ蛋白阴性标本中却有１９例（８２．６％）显示ｐ１６蛋白阳性或强阳性。结论（１）ｐ１６及Ｒｂ蛋白参与星形细胞瘤细胞增殖过程并影响其细胞分化；（２）ｐ１６与Ｒｂ蛋白之间阳性表达的相互抑制，可能是高度恶性星形细胞瘤的标志之一。

Relationship between p16 and Rb protein expression in astrocytomas

OBJECTIVE: p16 and Rb protein were examined in primary astrocytomas in order to study the correlation between p16 and Rb proteins. METHODS: p16 and Rb pretoin were immunostained by SP immunohistochemical method in the sections of formalin fixed paraffin embedded tumor tissue from 102 patients with astrocytoma brain tumors. RESULTS: p16 and Rb protein were expressed in all low grade (WHO Grade I and II) astrocytomas but only in 48.1% and 57.4% astrocytomas of Grade III and Grade IV respectively, in which, 24/31 of Rb protein positive tumors showed null or low expression of p16 protein, while 19/23 of Rb negative tumors were associated with positive or high level expression of p16 protein. CONCLUSIONS: (a) p16 and Rb proteins were both involved in astrocytoma progression. (b) Negatively correlated pressionof p16 and Rb protein might be one of characteristics of malignant astrocytoma.