影响肝细胞癌生物学行为及预后的相关因子分析

目的探讨影响肝癌生物学行为及预后的重要因素和寻求肝癌基因治疗的有效靶点。方法运用免疫组化方法检测Ki67、nm23H1、cMet及MT1MMP蛋白在93例肝细胞癌组织中的表达水平,并判断其与肝癌细胞恶性生物学行为及患者预后的关系。结果Ki67、nm23H1、cMet及MT1MMP蛋白表达水平与肝癌患者预后均具有显著相关性(P均<0.01);nm23H1的表达与肿瘤远处转移之间呈负相关关系(P=0.041);cMet、MT1MMP的表达与肝癌瘤旁浸润及远处转移之间均呈正相关关系(P<0.05)。结论nm23H1基因的失活或突变,伴随cMet以及MT1MMP蛋白的异常表达可能是导致肝癌浸润、转移的关键因素。

Analysis of the related factors which affect tumor biological behavior and prognosis in hepatocellular carcinoma

Purpose To investigate the factors which affect biological behavior and prognosis of hepatocarcinoma and hope to give a clue to new target for gene therapy on hepatocarcinoma. Methods The expressions of Ki-67, nm23-H1, c-Met, and MT1-MMP(membrane-type1 matrix metalloproteinase)protein were detected in 93 cases of hepatocellular carcinoma by means of immunohistochemistry and their correlations with clinicopathological factors were analyzed. Results The protein expressions of Ki-67, nm23-H1, c-Met, and MT1-MMP influenced the prognosis of hepatocellular carcinoma remarkably(P=0.009, P=0.000, P=0.008 and P=0.006 respectively). The protein expression of nm23-H1 showed a negative correlation with tumor distant metastasis(P=0.041). The protein expressions of c-Met and MT1-MMP had positive correlations with tumor envelope invasion and distant metastasis ( P<0.05). Conclusions The inactivation or mutation of nm23-H1 gene accompanied with the abnormal expression of c-Met and MT1-MMP proteins are key factors causing tumor invasion and metastasis.