FUNDC1调控肺癌A549细胞及其干细胞的生长、自噬、凋亡和细胞干性的研究

目的:本实验旨在阐明线粒体自噬受体蛋白FUNDC1(FUN 14 domain containing 1)在肺癌病理进程中对肿瘤细胞生长死亡以及细胞干性的调控作用.方法:使用RT-qPCR分析FUNDC1在肺癌组织和细胞中的表达情况.siRNAs和过表达载体转染A549细胞以及诱导形成的A549干细胞团(A549/CS...

FUNDC1 regulates the growth,autophagy,apoptosis and cell stemness of lung cancer A549 and its stem cells

Objective:To clarify the regulatory effects of FUNDC1(FUN 14 domain containing 1)exerted on the growth and death,as well as cell stemness,of tumor cells in the pathological process of lung cancer.Methods:The expression of FUNDC1 in lung cancer tissues and cells was analyzed by RT-qPCR.siRNAs and overexpression vectors were transfected into A549 cells and A549 cancer stem cells(A549/CSCs).Cell growth was analyzed using CCK-8 method and colony formation experiment.Western blotting was conducted to measure the expressions of FUNDC1,autophagy related proteins(Beclin-1,LC3-Ⅱ,p62),apoptosis related proteins(Bax,Bcl-2,caspase3)and stemness related proteins(CD133,Sox2,Oct4).Cell Death Elisa kit was used to determine the apoptosis rates.The rate of CD133+ cells was analyzed by Flow cytometry.Results:FUNDC1 expression was up-regulated in both lung cancer tissues and cells.Inhibiting FUNDC1 depressed the proliferation and protective autophagy of A549 cells,while apoptosis was promoted.Additionally,FUNDC1 was positive-correlated with CD133 expression,and FUNDC1 inhibition could significantly suppress CD133,Sox2,and Oct4.The expression of FUNDC1 in A549/CSC cells was elevated compared with normal A549 cells.FUNDC1 inhibition also decreased the proliferation and protective autophagy of A549/CSC cells and promote cell apoptosis.Conclusion:FUNDC1 regulates the growth,autophagy,apoptosis and cellular stemness of lung cancer cells as well as their CSC cells.