| 褪黑素抑制低氧引起大鼠大脑皮层氨基酸递质释放 |
| |
| 引用本文： | Gu J,Li XJ,Sun FY. 褪黑素抑制低氧引起大鼠大脑皮层氨基酸递质释放[J]. 生理学报, 1999, 51(4): 445-448 |
| |
| 作者姓名： | Gu J Li XJ Sun FY |
| |
| 作者单位： | 1. 上海医科大学基础医学院神经生物学教研室、医学神经生物学国家重点实验室,上海,200032
2. 上海医科大学基础医学院神经生物学教研室、医学神经生物学国家重点实验室,上海,200032;上海生命科学研究中心,上海,200031 |
| |
| 摘 要： | 为研究褪黑素对低氧引起大鼠大脑皮层脑片氨基酸释放变化的影响,利用反相高效液相色谱结合荧光检测法,测定了孵育液中氨基酸类神经递质的含量。低氧条件为通入９１．６％Ｎ２和８．４％Ｏ２的混合气体。低氧３０ｍｉｎ时,大鼠大脑皮层脑片孵育中,氨基酸类神经递质天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和γ－氨基丁酸的含量显著增加,其含量分别是正常氧组的２４０．４％,３３４．３％,２００．６％,２１０．４％,１６
|
| 关 键 词： | 褪黑素 低氧 脑片 兴奋性氨基酸 |
Melatonin decreases the release of amino acid transmitters in hypoxic rat cortical slice |
| |
| Gu J,Li X J,Sun F Y. Melatonin decreases the release of amino acid transmitters in hypoxic rat cortical slice[J]. Acta Physiologica Sinica, 1999, 51(4): 445-448 |
| |
| Authors: | Gu J Li X J Sun F Y |
| |
| Affiliation: | National Key Laboratory of Medical Neurobiology, Department of Neurobiology, Shanghai Medical University, Shanghai 200032. |
| |
| Abstract: | To study the effect of melatonin on changes in amino acid release following hypoxic stimulation, a RP-HPLC fluorometric method was used to measure the content of amino acids in the culture medium of rat brain slices. Hypoxic condition was instituted by a gas of 91.6% N2 and 8.4% O2. The results showed that the contents of amino acids, including aspartate, glutamate, glutamine, glycine, taurine and GABA, in the medium were significantly increased to 240.4%, 334.3%, 200.6%, 210.4%, 168.6% and 263.9%, respectively (P < 0.01 vs control) by 30 min hypoxic incubation. Melatonin can significantly reduce the increase of aspartate and glutamate, to 55.1% and 57.0% of the hypoxic group. |
| |
| Keywords: | melatonin hypoxia brain slice excitatory amino acids |
| 本文献已被 维普 万方数据 PubMed 等数据库收录！ |
|
