Binding of 5-methoxypsoralen to human serum low density lipoproteins |
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| Authors: | Teresa de Sa E Melo Patrice Morlière Sonia Goldstein René Santus Louis Dubertret Dominique Lagrange |
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| Affiliation: | 1. Laboratoire de Physico-Chimie de l''Adaptation Biologique Muséum National d''Histoire Naturelle 43 Rue Cuvier — 75231 Paris Cedex 05 France;2. Hôpital Henri Mondor Laboratoire de Recherche Dermatologique 94010 Creteil, France;3. Laboratoire de Biochimie B Faculté de Médecine St Antoine 27 Rue Chaligny — 75012 Paris, France;4. INSERM U.177 — Institut Biomédical des Cordeliers 15-21 Rue de l''Ecole de Médecine — 75006 Paris, France |
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| Abstract: | 5-methoxypsoralen (5-MOP) binds to human serum low density lipoproteins (LDL) according to a two-step process. Scatchard analysis of the first step yields K = 1.4 × 105 M?1 and 4 binding sites. It involves the LDL apoprotein. The second step corresponds to a solubilization, in the lipidic core, of ? 45 molecules of 5MOP per LDL molecule. It is accompanied by a large blue shift of the 5MOP fluorescence. The ability of LDL to bind 5MOP and to carry it into various cells may explain some biological effects sometimes encountered during PUVA therapy. |
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| Keywords: | 5-MOP 5-methoxypsoralen 8-MOP 8-methoxypsoralen L D L Low density lipoproteins |
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