蛋白质芯片技术应用于肝癌诊断的初步研究

 【摘要】　目的　应用表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）技术和蛋白质芯片从肝癌患者血清中筛选可用于肝癌诊断的标志蛋白质。方法　运用SELDI-TOF-MS技术及CM10蛋白质芯片检测46例原发性肝癌患者和64名健康人血清，获得蛋白质指纹图谱，采用Biomarker Wizard软件选出肝癌患者与健康人血清中的表达差异蛋白质，评价其灵敏度、特异度和诊断效能，确定最佳标志蛋白质。结果　肝癌组与健康对照组血清中有16个蛋白质的表达差异在2倍以上，并且差异有统计学意义（P＜0.05）。其中质荷比为6845.70的蛋白诊断效能最高，其灵敏度为89.1 %（41／46），特异度87.5 %（56／64），且该蛋白质与肿瘤大小有相关性（r＝-0.363，P＜0.05）。经数据库搜索该蛋白质很可能是免疫球蛋白重链可变区片段。结论　应用SELDI-TOF-MS技术诊断肝癌，具有灵敏度高，特异性好，快速简便的优点，质荷比为6845.70的蛋白质可能是肝癌患者血清中的特异性标志物。

Preliminary study of diagnosis ofhepatocellular carcinoma by protein chip technique

Obiective To screen serum biomarkers in patients with hepatocellular carcinoma (HCC)by SELDI-TOF-MS technique.Methods SELDI-TOF-MS technique and CM10 Protein Chip were used to detect serum protein patterns of 46 patients with primary hepatic carcinoma and 64 healthy persons.The different proteins were obtained by the Biomarker Wizard software between the patients and healthy persons.The best biomarker of primary hepatic carcinoma was selected by evaluating the sensitivity and specificity of the protein.Results 16 protein peaks were obviously different between the patients and the healthy persons (P ＜0.05).The protein peaks of m/z 6845.70 had the highest diagnosis value with a sensitivity of 89.1％ (41/46)and specificity of 87.5 ％ (56/64).This protein was likely to be a part of the immunoglobulin heavy chain variable region.Conclusion The protein of m/z 6845.70 is potential biomarkers for the diagnosis of HCC.SELDI-TOF-MS technique is a quick,simple,convenient and high through-put technology for diagnosis of hepatocellular carcinoma.