| Study on the binding mode of Mg（Sal2trien） with DNA |
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| 作者姓名： | XI Xiaoli YANG Manman ZHOU Chengyong ZHAO Jing YANG Pin |
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| 作者单位： | College of Chemistry and Chemical Engineering, Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Talyuan 030006, China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China （Grant No. 30470408）, and the Natural Science Foundation of Shanxi Province （Grant No. 20041032）. |
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| 摘 要： | Polyamide could not only combine with DNA out- side but also inhibit expressing of gene1]. At the same time, polyamide could coordinate many molecules. Ren et al.2] found that MgⅡ(dien)(OH)] can cleave pBR322DNA effectively in 2004. Liu et al.3,4] f…
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| 关 键 词： | 三亚乙基四胺 牛胸腺DNA 荧光光谱 循环伏安法 镁 |
| 收稿时间： | 2005-12-16 |
| 修稿时间： | 2005-12-162006-06-02 |
Study on the binding mode of Mg(Sal2trien) with DNA |
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| XI Xiaoli YANG Manman ZHOU Chengyong ZHAO Jing YANG Pin.Study on the binding mode of Mg(Sal2trien) with DNA[J].Chinese Science Bulletin,2006,51(19):2322-2326. |
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| Authors: | Xiaoli Xi Manman Yang Chengyong Zhou Jing Zhao Pin Yang |
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| Affiliation: | (1) College of Chemistry and Chemical Engineering, Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, China |
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| Abstract: | In this study the complex Mg(Sal2trien) was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra,
fluorescence spectra, viscosity and CV (cyclic voltammetry). It was found that after the complex acted with CT DNA, the Abs
of UV spectra rose obviously; the fluorescence intensity of EB-DNA was almost not changed; viscosity decreased. Determination
of cyclic voltammetry showed that DNA made the MgL’s formal potential negatively shift. Scatchard plot showed that the addition
of the binding mode of the complex to EB was uncompetitive inhibition compared with EB to DNA. So the binding mode of the
complex with CT DNA was stable-electricity binding. Then the interaction of the complex with pBR322 was studied by the method
of gel electrophoresis. The result showed that the complex could cleave pBR322 DNA effectively. |
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| Keywords: | Mg(Sal_2trien) calf thymus DNA Scatchard plot |
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