高效液相色谱法同时测定食品中的12种抗氧化剂

目的:建立一种快速、准确测定食品中12种抗氧化剂的高效液相色谱法。方法:样品用正己烷溶解,用含抗坏血酸棕榈酸盐(AP)的饱和乙腈萃取,以反相C18柱为分离柱,以甲醇-乙腈-乙酸-水体系为流动相进行梯度洗脱,采用高效液相色谱-紫外检测器于280nm定量检测。结果:12种抗氧化剂在36min内完全分离,线性范围为0.2～200mg/L(r=0.9981～0.9999),定量限为0.2～1.0mg/kg,回收率为81.13%～107.57%,相对标准偏差为0.26%～4.52%(n=7)。结论:本方法准确、可靠、简便、检出限低,适合分析大批量样品。

Simultaneous Determination of 12 Antioxidants in Foods by High-performance Liquid Chromatography

This study aimed to present a high-performance liquid chromatographic(HPLC) method for the determination of 12 antioxidants in foods.Samples were dissolved in n-hexane saturated with acetonitrile and extracted with the lower phase(containing ascorbyl palmitate) resulting from n-hexane saturated with acetonitrile.A C18 column and a mobile phase consisting of methanol,acetonitrile,acetic acid and water were used for the chromatographic separation in the gradient elution mode.The detection wavelength of UV det...