| 灭活前后HIV-1包膜基因变异的研究 |
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| 引用本文： | 樊卫平,李敬云,王红霞. 灭活前后HIV-1包膜基因变异的研究[J]. 山西医科大学学报, 2000, 31(4): 294-296 |
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| 作者姓名： | 樊卫平 李敬云 王红霞 |
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| 作者单位： | [1]山西医科大学微生物免疫学教研室 [2]军事医学科学院微生物流行病学研究所 |
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| 摘 要： | 目的 深入了解ＨＩＶ在灭活因素作用下包膜基因变异。方法 对ＨＩＶ－１Ｂ３亚型毒株在Ｓ／Ｄ法及低ｐＨ法灭活作用前后的样品，套式ＰＣＲ扩增其民膜基因Ｃ２￣Ｃ３区５６４ｂｐ的核酸自段，进行核苷酸序列测定和分析。结果 两种方法活前后，包膜基因变异均不显著，核苷酸同源性均为９８％。结论 Ｓ／Ｄ法及低ｐＨ法灭活作用前后ＨＩＶ包膜基因变异不显著。体外传代ＨＩＶ包膜基因趋于稳定。
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| 关 键 词： | HIV 艾滋病毒 包膜基因变异 血液制品 灭活病毒 |
Variation of HIV-1 env gene by inactivators |
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| FAN Wei ping,LI Jing yun,WANG Hong xia,et al. Variation of HIV-1 env gene by inactivators[J]. Journal of Shanxi Medical University, 2000, 31(4): 294-296 |
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| Authors: | FAN Wei ping LI Jing yun WANG Hong xia et al |
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| Abstract: | Objective To thoroughly understand the variation of HIV-1 env gene under inactivators. Methods Taking the HIV before and after S/D,acid's exerting as the objects, env gene C2-C3 region 564 bp fragment of these HIV was obtained by nest PCR, then sequenced and analyzed. Results Before and after the two inactivators exerting,all the env gene variation was indistinctive,necleotide homogeneity was all 98%. Conclusion Before and after the two inactivators exerting, the variation of HIV-1 env gene is indistinctive. Serial passaged HIV env gene tends to stablility. |
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| Keywords: | HIV virus inactivated variation (genetics) |
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