水通道蛋白3基因敲除小鼠的饲养繁殖及鉴定

目的: 繁殖和鉴定水通道蛋白3(AQP-3)基因敲除小鼠。方法: 将引进的杂合子小鼠进行饲养并繁殖,繁殖成功后其子代中将会出现野生型、杂合子以及纯合子3种基因型,提取每种基因型小鼠尾部基因组DNA,用PCR法进行鉴定,将其雄性纯合子与雌性杂合子交配,依照孟德尔遗传定律就有可能获得较多的基因敲除纯合子小鼠。结果: AQP-3杂合子小鼠的繁殖和饲养均获得成功,亦获得了较多的基因敲除纯合子小鼠。结论: 正确的饲养繁殖以及鉴定方法是从杂合子中获得AQP-3基因敲除纯合子小鼠的有效途径。

Breeding,reproducing and identifying for aquaporin-3 gene knock-out mice

Objective: To breed and identify aquaporin-3 (AQP-3) gene knock-out mice. Methods: Heterozygote mice were bred and reproduced. Wild genotype, heterozygote genotype and homozygote genotype would appear in the offsprings of parents. Genome DNA extracted from the murine tails was subjected to PCR for genotype identification. Male homozygote mice were selected to mate with the female heterozygote mice to acquire homozygote baby mice according to Mendel law. Results: The breeding and reproducing were successful and more heterozygote genotype mice were reproduced. Conclusions: Appropriate methods of breeding, reproducing and identifying are effective ways for acquiring AQP-3 gene knock-out mice from heterozygote mice.