Rational design of sequestered DNAzyme beacons to enable flexible control of catalytic activities |
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| Authors: | Wei Tang Mengxi Cheng Danling Dai Zhonghua Xiong Feng Liu |
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| Affiliation: | Institute of Materials, China Academy of Engineering Physics, Mianyang 621700 China.; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 China |
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| Abstract: | DNAzymes as functional units play increasingly important roles for DNA nanotechnology, and fine control of the catalytic activities of DNAzymes is a crucial element in the design and construction of functional and dynamic devices. So far, attempts to control cleavage kinetics can be mainly achieved through varying the concentrations of the specific metal ions. Here we present a facile sequestered DNAzyme beacon strategy based on precisely blocking the catalytic core of the DNAzyme, which can flexibly regulate the DNAzyme cleavage kinetics without changing the concentrations of metal ions. This strategy can be extended to couple with a large number of other RNA-cleaving DNAzymes and was successfully applied in designing a dual stem-loop structure probe for arbitrary sequence biosensing, which provides the possibility of scaling up versatile and dynamic DNA devices that use DNAzymes as functional modules.We present a sequestered DNAzyme beacon strategy based on precisely blocking the catalytic core for flexible regulation of DNAzyme kinetics. |
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