Effect of alkylated and intercalated DNA on the generation of superoxide anion by riboflavin |
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Authors: | Imrana Naseem Maqbool Ahmad S M Hadi |
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Affiliation: | (1) Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, 202002 Aligarh, India |
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Abstract: | Superoxide anion (O
2
.–
) was photogenerated upon illumination of riboflavin in fluorescent light. The rate of O
2
.–
formation was stimulated by double stranded DNA but not by denatured DNA or RNA. Depurinated DNA, which was predominantly depleted in guanine residues, did not exhibit the stimulatory effect, indicating an interaction of riboflavin, or active oxygen species derived from it, with guanine bases. Also, the stimulation of O
2
.–
photogeneration was not observed with ethidium bromide but was seen with proflavin-intercalated DNA. Since ethidium bromide intercalates preferentially between purines and pyrimidines, and proflavin prefers dA-dT rich sites, these results were interpreted to suggest that the interaction of riboflavin with DNA is mainly with GC or CG base pairs. |
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Keywords: | riboflavin superoxide anion alkylated and intercalated DNA |
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