Surface plasmon resonance imaging (SPRi) as an alternative technique for rapid and quantitative screening of small molecules, useful in drug discovery |
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Authors: | Flavien PilletAuthor Vitae,Carmen RomeraAuthor Vitae,Emmanuelle Tré visiolAuthor Vitae,Sophie BellonAuthor VitaeMarie-Paule Teulade-FichouAuthor Vitae,Jean-Marie Franç oisAuthor Vitae,Geneviè ve PratvielAuthor Vitae,Vé ronique Anton LeberreAuthor Vitae |
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Affiliation: | a Université de Toulouse, INSA,UPS,INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France b INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France c CNRS, UMR5504, F-31400 Toulouse, France d Horiba Scientific-Genoptics, Bat 503, Plateau du Moulon, F-91401 Orsay, France e Laboratoire de Chimie de Coordination, CNRS, 205 route de Narbonne, 31077 Toulouse cedex4, France f Université de Toulouse, Toulouse, France g Institut Curie, UMR 176, Centre Universitaire, 91405 Orsay, France |
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Abstract: | Surface plasmon resonance imaging (SPRi) is a label free technology for biomolecular interaction, which gives access to binding kinetic parameters from real time acquisition. It offers the possibility to test in a single run a large number of interactions, allowing rapid identification of the most suitable compounds toward a given biological entity. Until now, this technique has proven to be relevant for interaction between relatively large molecules (protein, antibodies, DNA) but has not been challenged yet for the screening of small molecules that can be of interested in the field of drug discovery. As a proof a principle, we have used SPRi to screen for interaction of several small molecules, referred to as G4-ligands on G-quadruplex DNA. This technology allowed to easy discrimination of the binding properties of four G4-ligands on quadruplex DNA models. |
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Keywords: | Surface plasmon resonance imaging (SPRi) Drug discovery Quadruplex binding |
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