2-巯基苯并咪唑的表面增强拉曼光谱检测方法研究

以表面增强拉曼光谱(SERS)方法对2-巯基苯并咪唑(2-MBI)进行了研究,以自组装在玻璃基片上的银纳米膜作为SERS增强基底,采集了2-MBI的SERS光谱图,并对其拉曼特征峰进行了指认。研究了吸附时间和分子浓度对拉曼光谱的影响,以411cm~(-1)拉曼谱峰为定性和定量分析的特征峰。在10-6~10-3mol·L~(-1)浓度范围内拉曼光谱强度与2-MBI浓度的负对数呈现较好的线性关系,线性方程为I=1 237.8logc+8 326.3,线性相关系数为0.999 8,相对标准偏差在0.025~0.084之间,此方法检测2-MBI的检测限为10-7 mol·L~(-1)。这些研究为发展新的针对2-MBI的检测方法奠定了基础。

Enhanced Raman Spectroscopy

In this study the surface enhanced Raman spectroscopy (SERS) was employed to study the detection of 2-MBI .The self-assembly silver nanoparticle films on glass slides were used as SERS substrates .SERS spectra were obtained and the charac-teristic bands were assigned .The time and concentration dependent experiments were conducted and finally the optimized experi-mental conditions were confirmed to achieve the qualitative and quantitative analysis .The SESR band at 411cm -1was selected as the characteristic peak to do the qualitative and quantitative analysis .The results show that there was a linear correlation be-tween the band intensity and the minus logarithm of concentration in the range of I=1237.8logc+8 326.3 ,while the linear cor-relation coefficient was 0.999 8 .The relative standard deviation was between 0.025~0.084 .The detection limit was 10-7 mol · L -1.It is concluded that this research has a great potential application in 2-MBI detection .