| 人抵抗素基因cDNA的克隆 |
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| 引用本文： | 周剑,王佑民,王长江,章秋,胡红琳,时照明.人抵抗素基因cDNA的克隆[J].安徽医科大学学报,2004,39(2):119-122. |
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| 作者姓名： | 周剑 王佑民 王长江 章秋 胡红琳 时照明 |
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| 作者单位： | 安徽医科大学第一附属医院内分泌科,合肥,230022 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 目的克隆人抵抗素基因(hRETN)cDNA,为进一步研究RETN的结构和功能提供实验基础.方法应用RT-PCR方法从中国人网膜脂肪垫总RNA中扩增出RETN 基因cDNA,克隆入载体pMD18-T中,形成重组载体pMD18-T/hRETN.通过蓝白斑筛选出阳性克隆,限制性内切酶酶切鉴定后对其进行测序.结果从脂肪组织总RNA中扩增得到363 bp片段hRETN基因,其cDNA序列与Genbank hRETN基因序列基本相同.结论成功地克隆中国人hRETN cDNA.
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| 关 键 词： | 脂肪组织/代谢 -克隆 分子 -氨基酸序列 -DNA 互补 |
| 文章编号： | 1000-1492(2004)02-0119-04 |
| 修稿时间： | 2003年11月5日 |
cDNA cloning of complete resistin gene coding sequence from Chinese |
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| Zhou Jian,Wang Youmin,Wang Changjiang et al.cDNA cloning of complete resistin gene coding sequence from Chinese[J].Acta Universitis Medicinalis Anhui,2004,39(2):119-122. |
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| Authors: | Zhou Jian Wang Youmin Wang Changjiang |
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| Abstract: | Objective To provide experimental basis for further research on resistin function,human resistin gene (hRETN) was cloned. Methods hRETN mRNA from omentum fat pat of Chinese was amplified by RT-PCR and cloned into plasmid pMD18-T to generate the recombinant plasmid pMD18-T/hRETN,which was confirmed to contain the amplified target gene segments by digest of restrictive endonuclease and nucleotide sequencing. Results The sequencing results for amplified target gene showed that the sequence of hRETN from omentum fat pat of Chinese was identical to that of human RETN in Genbank. Conclusion hRETN cDNA is successfully cloned from Chinese. |
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| Keywords: | adipose tissue/metabolism clone molecular amino acid sequence DNA complementary |
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