Fibulin-5在醛固酮腺瘤模型大鼠主动脉中的表达

目的 观察醛固酮对主动脉Fibulin-5表达的影响.方法 将32只SD大鼠随机分为4组,每组8只:(1)空白溶剂设为对照(60%丙二醇+10%乙醇+30%双蒸水);(2)醛固酮腺瘤:泵入醛固酮(1 μg/h);(3)醛固酮腺瘤+特异性盐皮质激素受体阻断剂:依普利酮[100 mg/(kg·d)];(4)醛固酮腺瘤+血管扩张剂:肼苯哒嗪[25 mg/(kg·d)].渗透泵内分别注入醛固酮或空白溶剂,然后将其埋植于大鼠背部皮下.8周后,免疫组织化学和免疫印迹法检测主动脉Fibulin-5的表达.结果 与对照组大鼠比较,腺瘤组大鼠主动脉Fibulin-5的表达显著增加(P＜0.05);与肼苯哒嗪不同,依普利酮可以抑制醛固酮的上述作用(P＜0.05).结论 醛固酮可以直接诱导主动脉Fibulin-5 表达增加,后者在醛固酮增多引起的主动脉重构过程中可能发挥重要作用.

Fibulin-5 expression in the aortas of model rats with aldosterone-producing adenomas

Objective To observe the effect of aldosterone on fibulin-5 expression in the aorta.Methods Thirty-two male rats were randomly divided into 4 groups;vehicle (control) ,aldosterone-producing adenoma, aldosterone-producing adenoma plus eplerenone or hydralazine. They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle. After 8 weeks, Fibulin-5 was examined by immnuohistochemistry and Western blotting. Results Compared with controls, the model rats with aldosterone-producing ademoma exhibited elevated aortic expression of Fibulin-5. This effect of aldosterone was significantly suppressed after co-treatment with eplerenone but not with hydralazine. Conclusion It suggest that aldosterone directly promotes aortic expression of Fibulin-5 that may play an important role in aldosterone-induced aortic remodeling.