| 离子影像系统测定分离的单个心肌细胞钙瞬变和收缩 |
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| 引用本文： | 崔香丽,周尚忠,吴博威. 离子影像系统测定分离的单个心肌细胞钙瞬变和收缩[J]. 山西医科大学学报, 2002, 33(5): 461-463 |
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| 作者姓名： | 崔香丽 周尚忠 吴博威 |
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| 作者单位： | 1. 山西医科大学生理学教研室,太原,030001
2. 大同市环境监测站 |
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| 摘 要： | 目的 建立一种单细胞钙瞬变的测定方法。方法 选用正常和心肌肥厚Wistar大鼠，分离出心肌细胞，用新购进的离子影像分析系统进行钙瞬变和收缩的测定。结果和结论 该系统可用于同步测定钙瞬变和细胞收缩。
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| 关 键 词： | 心肌 fura－2 心肌收缩 钙瞬变 离子影像 大鼠 |
| 文章编号： | 1007-6611(2002)05-0461-03 |
Measurement of Ca2 + transient and contraction of single cardiomyocytes using ion imaging system |
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| CUI Xiang li,ZHOU Shang zhong,WU Bo wei. Measurement of Ca2 + transient and contraction of single cardiomyocytes using ion imaging system[J]. Journal of Shanxi Medical University, 2002, 33(5): 461-463 |
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| Authors: | CUI Xiang li ZHOU Shang zhong WU Bo wei |
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| Abstract: | Objective To establish a method to detect the Ca 2+ transient in single cells. Methods After isolation of cardiac myocytes from normal and hypertrophied rat hearts, Ca 2+ transient and contraction were measured using ion imaging system that was purchased newly. Results and Conclusion The system could be used to measure Ca 2+ transient and cell contraction simultaneously. |
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| Keywords: | myocardium fura 2 myocardial contraction Ca 2+ transient ion imaging rats |
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