| 免疫应激溃疡性结肠炎模型的建立与评价 |
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| 引用本文： | 李楠,;王雪明,;稽杨,;张林,;张虹,;宋晶莹,;石玉玲. 免疫应激溃疡性结肠炎模型的建立与评价[J]. 解放军医学高等专科学校学报, 2008, 0(4): 496-498 |
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| 作者姓名： | 李楠, 王雪明, 稽杨, 张林, 张虹, 宋晶莹, 石玉玲 |
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| 作者单位： | [1]解放军总医院第二附属医院消化科,北京100091; [2]解放军总医院第二附属医院药剂科,北京100091; [3]解放军总后勤部药物鉴定所,北京100091; [4]解放军总医院第二附属医院病理科,北京100857 |
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| 摘 要： | 目的探讨用联合免疫噪声应激法建立大鼠溃疡性结肠炎模型的可行性及价值。方法将80只Wister大鼠随机分为A、B、C、D四组,每组20只。A组为正常对照组;B组为免疫造模组;C组为噪声应激组;D组为免疫＋噪声应激联合组。比较造模后20d各组血清皮质醇、结肠组织中MPO、NO水平和结肠组织损伤评分。结果联合免疫噪声应激法较免疫组、噪声应激组的结肠组织损伤评分、血清皮质醇、结肠组织MPO、NO含量均明显增高。结论联合免疫噪声应激法建立的溃疡性结肠炎模型持续时间长,优于单一因素,是一种有价值的可行造模方法。
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| 关 键 词： | 免疫复合法 溃疡性结肠炎 模型 噪声 激素 |
Foundation and Appreciation of Ulcerative Colonitis Model by Combining Immunizatione with Stress |
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| Affiliation: | Li Nan,Wang Xue-ming,Ji Yang,Zhang Lin,Zhang Hong,Song Jing-ying,Shi Yu-ling (1. Department of Gasterology; 2. Department of Pharmacy; 3. Department of Pathology,Second Affiliated Hospital of PLA General Hospital,Beijing 100091,China; 4. Pharmaceutical Identification Institute of PLA General Logistic Section,Beijing 100857,China) |
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| Abstract: | Objective To found and appreciate ulcerative colonitis model by combining immunization with stress. Methods A total of 80 Wister rats were divided into four groups （20 rats per group）, i.e. Group A （normal control group）, Group B （immunization model group）, Group C （noise stress model group） and Group D （immunization plus noise stress model group）. Results Tissue lesion score, serum cortisol, tissue myeloperoxidase （MPO） and NO were obviously higher in Group D than in Group B or C.Conclusion The ulcerative colonitis model founded by combining immunization with stress is feasible and better than any of other models. |
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| Keywords: | ulcerative colonitis model immunization noise stress |
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