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| 中国新疆紫花苜蓿复合体３个种的遗传多样性及亲缘关系研究 | |
| 引用本文： | 李飞飞,崔大方,羊海军,邓超宏,李庆艳. 中国新疆紫花苜蓿复合体３个种的遗传多样性及亲缘关系研究[J]. 草业学报, 2012, 21(1): 190-198. DOI: 10.11686/cyxb20120125 |
| 作者姓名： | 李飞飞 崔大方 羊海军 邓超宏 李庆艳 |
| 作者单位： | 1. 华南农业大学生命科学学院,广东广州,510642 2. 华南农业大学林学院,广东广州510642;华南农业大学公共基础实验教学中心,广东广州510642 3. 华南农业大学公共基础实验教学中心,广东广州,510642 |
| 摘 要： | 利用１５对ＳＳＲ引物和１０个ＩＳＳＲ引物对紫花苜蓿复合体３个种黄花苜蓿、多变苜蓿及紫花苜蓿共１０个居群的遗传多样性及亲缘关系进行研究。结果表明，ＳＳＲ标记下，１０个居群中多变苜蓿ＶＧ 居群多态位点百分率、Ｓｈａｎｎｏｎ信息指数及Ｎｅｉ’ｓ基因多样性指数均最高；ＩＳＳＲ标记下，黄花苜蓿ＦＨ居群以上３个指数均最高；黄花苜蓿居群ＦＨ在２种分子标记下都存在特有位点。说明在１０个居群中多变苜蓿居群ＶＧ与黄花苜蓿居群ＦＨ表现出较丰富的遗传多样性，值得进一步保护。ＵＰＧＭＡ聚类分析结果表明，在２种分子标记下３个种可以被有效区分，结合它们在新疆境内的分布，本研究支持仍将紫花苜蓿、黄花苜蓿和多变苜蓿划分为３个种。 |
| 关 键 词： | 紫花苜蓿复合体 遗传多样性 亲缘关系 |
Genetic diversity and genetic relationships among three species of Medicago sativa complex in Xinjiang Province of China |
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| LI Fei-fei , CUI Da-fang , YANG Hai-jun , DENG Chao-hong , LI Qing-yan. Genetic diversity and genetic relationships among three species of Medicago sativa complex in Xinjiang Province of China[J]. Acta Prataculturae Sinica, 2012, 21(1): 190-198. DOI: 10.11686/cyxb20120125 | |
| Authors: | LI Fei-fei CUI Da-fang YANG Hai-jun DENG Chao-hong LI Qing-yan |
| Affiliation: | 1.College of Life Sciences,South China Agricultural University,Guangzhou 510642,China;2.College of Forestry,South China Agricultural University,Guangzhou 510642,China;3.Center of Experimental Teaching for Common Basic Courses,South China Agricultural University,Guangzhou 510642,China) |
| Abstract: | The genetic diversity and the genetic relationships among 10 populations of Medicago falcata,M.varia and M.sativa,belonging to the M.sativa complex were studied by using 15 pair of SSR primers and 10 ISSR primers.The results showed that population VG of M.varia had the highest percentage of polymorphism bands(PPB),Shannon’s information index(I) and Nei’s genetic diversity index(h) of 10 populations in SSR results;population FH of M.falcata had the 3 highest indices in ISSR results;population FH had private bands in SSR and ISSR results,which indicated VG of M.sativa and FH of M.falcata had the highest genetic diversity and thus need to be protected further.Three species could be distinguished clearly by UPGMA cluster analysis and combined their distribution in Xinjiang area,our study supported their classification at the species level. |
| Keywords: | Medicago sativa complex genetic diversity genetic relationship |
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