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婴幼儿配方粉生产中克罗诺杆菌溯源研究
引用本文:苏秀敏,张艳,陈佳,陈进,秦明倩,甘辛,李凤琴,杨保伟.婴幼儿配方粉生产中克罗诺杆菌溯源研究[J].中国乳品工业,2022(1):19-23.
作者姓名:苏秀敏  张艳  陈佳  陈进  秦明倩  甘辛  李凤琴  杨保伟
作者单位:西北农林科技大学食品科学与工程学院;富平县检验检测中心(陕西省羊乳产品质量监督检验中心);石家庄学院化工学院;国家食品安全风险评估中心
基金项目:克罗诺杆菌在婴幼儿配方粉生产过程中的迁移检测(K3040216007)。
摘    要:结合陕西省两个婴幼儿配方乳粉加工厂婴配粉生产工艺,对生产原辅料、车间环境、加工设备及工厂周边土壤进行采样,检测克罗诺杆菌的污染状况,并对菌株进行脉冲场凝胶电泳(Pulse field gel electrophoresis,PFGE)分型溯源,定位克罗诺杆菌在生产过程的污染点.结果 表明,采集的1246份样品中检出14...

关 键 词:婴幼儿配方粉  克罗诺杆菌  脉冲场凝胶电泳  溯源

Research on Traceability of Cronobacter in the Production of Powdered Infant Formula
SU Xiumin,ZHANG Yan,CHEN Jia,CHEN Jin,QIN Mingqian,GAN Xin,LI Fengqin,YANG Baowei.Research on Traceability of Cronobacter in the Production of Powdered Infant Formula[J].China Dairy Industry,2022(1):19-23.
Authors:SU Xiumin  ZHANG Yan  CHEN Jia  CHEN Jin  QIN Mingqian  GAN Xin  LI Fengqin  YANG Baowei
Affiliation:(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;Fuping Inspection and Testing center,Shaanxi Goat Milk Product Quality Supervision and Inspection Center,Fuping 711700,China;College of Chemical Technology,Shijiazhuang University,Shijiazhuang 050035,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China)
Abstract:In this study,according to the production procedure of two powdered infant formula(PIF)processing plants in Shaanxi Province,raw materials,workshop environment,processing equipment and the soil around the factory were sampled to investigate the prevalence of Cronobacter spp.The Cronobacter isolates were further subtyped via pulse field gel electrophoresis(PFGE)to trace the contamination points of Cronobacter spp.during PIF processing.Fourteen(1.12%)Cronobacter spp.positive samples were detected in 1246 samples collected previously and 33 Cronobacter isolates were recovered.The 33 isolates were subtyped into 14 genotypes with the similarity of 85.9%-100.0%.Combining the PFGE subtyping results and milk powder processing procedure,we conclude that the lactose powder,the staff sole,the soil around factory,the package workshop,the air inlet and purification system,the workshop gutter might be the potential key Cronobacter contamination points.The results of this study provide basic information for Cronobacter contamination sources identification and control during PIF production.
Keywords:powdered infant formula  Cronobacter spp    pulse field gel electrophoresis  contamination trace
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