假喜马拉雅块菌化学成分分析及其抗氧化能力研究

通过超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(ultra-performance liquid chromatography-tan-dem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometr...

The analysis of the antioxidant activities and chemical constituents of Tuber pesudohimalayense

Through ultra-performance liquid chromatography-tandem q-exactive quadrupole-electrostatic field track trap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS HRMS)analysis and in vitro antioxidant activity(DPPH radicals,ABTS cationic radicals,hydroxyl scavenging rate,reducing power and total phenolic content)determination,the main nutrients of the 80%methanol extract of the Tuber pesudohimalayense and the antioxidant capacity of different solvents of the fruiting body(80%methanol,water)extract were preliminarily studied.A total of 51 components were identified from T.pesudohimalayense,including 13 free amino acids,11 organic acids,8 nucleosides,9 amides,and 10 other types,8 components(phenylalanine,leucine,L-glutamic acid,L-tyrosine,adenosine,benzoic acid,oleamide,pyrazine)were unambiguously identified by comparing with reference standards.The antioxidant results showed that the water extract(H₂O)had higher scavenging and reducing power activities against DPPH free radicals,ABTS free radicals,and hydroxyl free radicals.The EC₅₀ values were 14.97,0.19,3.21 mg/mL,respectively.The total phenol content of the water extract(H₂O)was the highest(3.99 mg/g),and the total phenol content of the methanol extract(ME)was 1.87 mg/g.The study showed that the total phenol content and antioxidant activity in different extracts of truffles were different,and the total phenol content and antioxidant activity showed a positive correlation.This research could lay the foundation for the quality evaluation of truffles,the basic research of chemical composition and pharmacology,which providing a scientific basis for the development of natural antioxidants,and guiding significance for the development and utilization of truffle resources.