EFFECTS OF SeO_2 ON REGULATORY REGIONS p250 OF c-fos GENE

The element selenium was recognized 40 years ago as being essential in the nutrition of animal and humans and it is an essential component of a number of enzymes present in the amino acid selenocysteine (Se-Cys). Selenium has also been shown to inhibit tumorigenesis in both animal models and humans1]. Supplementing with selenium in diets may reduce the human cancer risk2]. It has been reported that cell protein kinase cdk2 andprotein kinase C were strongly inhibited by various forms of sel…

EFFECTS OF SeO2 ON REGULATORY REGIONS p250 OF c-fos GENE

Objective: This study was designed to determinewhether regulatory regions p250 of c-fos gene wereresponsive to SeO2 and to seek the possible mechanisms of regulation. Methods: HeLa cells were transfected withplasmids p250-tk CAT containing upstream regulatingregions of c-fos gene. Cells were treated by SeO2 for 20 min. CAT expression in transfected cells was observed by thinlayered chromatography. Results: In transfected HeLa cells CAT expression showed obvious increase after exposure to SeO2, especially in 10 mmol/L and 30 mmol/L group (P<0.05). Conclusion: Through affecting regulatory regions p250 of c-fos gene, SeO2 exerted biological effect on tumor cells. SeO2possibly had anti-tumor effects.