| 应用抑制性消减杂交法筛选与红曲菌产桔霉素相关的基因 |
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| 引用本文： | 赖卫华,许杨,熊勇华,李燕萍.应用抑制性消减杂交法筛选与红曲菌产桔霉素相关的基因[J].食品科学,2005,26(3):63-66. |
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| 作者姓名： | 赖卫华 许杨 熊勇华 李燕萍 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,江西,南昌,330047 |
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| 基金项目： | 国家自然科学基金(30460006)，江西省教育厅项目(2001)，江西省社会发展攻关课题(200309) |
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| 摘 要： | 为了筛选与红曲菌产桔霉素相关的基因，应用抑制性消减杂交(SSH)构建了两个红曲菌的cDNA消减文库。利用分子生物学软件从这两个文库中找出八条同源序列，通过Blast软件对这八条序列进行分析，结果显示，它们可能是新的基因片段，递交后被Genbank接收。
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| 关 键 词： | 抑制性消减杂交 基因 Blast软件 筛选 cDNA消减文库 红曲 分子生物学 桔霉素 |
| 文章编号： | 1002-6630(2005)03-0063-04 |
Screening Correlative Genes from Citrinin Biosynthetic Pathway in Monascus Species of by SSH |
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| LAI Wei-hua,XU Yang,XIONG Yong-hua,LI Yan-ping.Screening Correlative Genes from Citrinin Biosynthetic Pathway in Monascus Species of by SSH[J].Food Science,2005,26(3):63-66. |
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| Authors: | LAI Wei-hua XU Yang XIONG Yong-hua LI Yan-ping |
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| Abstract: | Two cDNA subtractive libraries of Monascus were constructed by suppression subtractive hybridization(SSH) and screened of correlative genes from the citrinin biosynthetic pathway in Monascus species. All clones in the two libraries were sequenced and the results were analyzed by bioinformatic softwares. There were 8 homologous ESTs in both libraries. Comparing with other ESTs by BLAST, these ESTs were novel, and were accepted by Genbank. |
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| Keywords: | Monascus citrinin SSH subtractive cDNA library |
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