液相色谱-原子荧光光谱法测定三七花中亚砷酸根和砷酸根含量的不确定度评价

目的 评定液相色谱-原子荧光光谱法(high performance liquid chromatography-atomic fluorescence spectrometry, HPLC-AFS)测定三七花中亚砷酸根和砷酸根含量(以As计)的不确定度。方法 根据JJF 1059.1- 2012《测量不确定度评定与表示》对各不确定度进行分析、确认及计算, 合成扩展不确定度。结果 三七花中亚砷酸根的平均值为0.1314 mg/kg, 相对标准偏差为1.23%, 扩展不确定度为0.0097 mg/kg, 砷酸根的平均值为0.1467 mg/kg, 相对标准偏差为3.02%, 扩展不确定度为0.0144 mg/kg。结论 在测定三七花中亚砷酸根和砷酸根含量过程中, 不确定度主要来源于标准曲线测定及拟合过程。

Evaluation of uncertainty of arsenite and arsenate content in the flower of Panax notoginseng by high performance liquid chromatography-atomic fluorescence spectrometry

Objective To evaluate the uncertainty in determination of arsenite and arsenate (As) content in the flower of Panax notoginseng by high performance liquid chromatography-atomic fluorescence spectrometry (HPLC- AFS). Methods According to JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement, the measurement uncertainty was analyzed, determined and evaluated, the combined expanded uncertainty was calculated. Results The average content of arsenite was 0.1314 mg/kg, the relative standard deviation was 1.23% and the extended uncertainty was 0.0097 mg/kg. The average content of arsenate was 0.1467 mg/kg, the relative standard deviation was 3.02% and the extended uncertainty was 0.0144 mg/kg. Conclusion In the process of the determination of arsenite and arsenate content in the flower of Panax notoginseng, the uncertainty are mainly derived from the determination and fitting of the standard curve.