| 同源序列对CYP2A13基因SNP研究的影响 |
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| 引用本文： | 滑峰,万海粟,梅朝蓉,郑德杰,孙琳琳,陈军,刘红雨,周清华.同源序列对CYP2A13基因SNP研究的影响[J].中国肺癌杂志,2010,13(2). |
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| 作者姓名： | 滑峰 万海粟 梅朝蓉 郑德杰 孙琳琳 陈军 刘红雨 周清华 |
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| 作者单位： | 天津医科大学总医院,天津市肺癌研究所,天津市肺癌转移与肿瘤微环境重点实验室,天津,300052 |
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| 基金项目： | 国家“十一五”科技攻关项目(No.2006BAI02A01);;国家“863”项目(No.2006AA02401);;天津市科技支撑计划中瑞合作重大项目(No.09ZCZDSF04100)项目资助~~ |
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| 摘 要： | 背景与目的已有研究表明:细胞色素P450酶2A13(cytochrome P4502A13,CYP2A13)在单核苷酸多态性(single nucleotide polymorphisms,SNP)与疾病关联中起重要作用。细胞色素P450酶是一组同工酶,基因序列间有高度的同源性,可能对SNP分析产生影响。本研究初步探讨同源序列对CYP2A13基因SNP研究的影响。方法应用Taqman探针检测573例人群rs8192789位点的分布,采用BLAST方法分析引物结合序列。对60例样本的CYP2A13序列进行测序,进一步进行了TA克隆测序,应用BLAST方法分析了克隆测序结果。结果rs8192789位点在573例人群中只有3例为TT纯合型,其余均为CT杂合型,BLAST分析为同源序列导致。60例人群CYP2A13部分序列测序结果完全一致,有大量套峰,101氨基酸位点处没有dbSNP数据库中报道的SNP位点。克隆测序为247bp、235bp两个片段。结论CYP2A13的同源序列对SNP研究造成了干扰,部分SNP位点可能是不存在的。
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| 关 键 词： | 细胞色素P450代谢酶2A13 单核苷酸多态性 同源序列 |
Interference of Homologous Sequences on the SNP Study of CYP2A13 Gene |
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| Feng HUA,Haisu WAN,Chaorong MEI,Dejie ZHENG,Linlin SUN,Jun CHEN,Hongyu LIU,Qinghua ZHOU.Interference of Homologous Sequences on the SNP Study of CYP2A13 Gene[J].Chinese Journal of Lung Cancer,2010,13(2). |
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| Authors: | Feng HUA Haisu WAN Chaorong MEI Dejie ZHENG Linlin SUN Jun CHEN Hongyu LIU Qinghua ZHOU |
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| Abstract: | Background and objective It has been proven that cytochrome P450 enzyme 2A13 (CYP2A13) played an important role in the association between single nucleotide polymorphisms (SNP) and human diseases.Cytochrome P450 enzymes are a group of isoenzymes,whose sequence homology may interfere with the study for SNP.The aim of this study is to explore the interference on the SNP study of CYP2A13 caused by homologous sequences.Methods Taqman probe was applied to detect distribution of rs8192789 sites in 573 subjects,an... |
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| Keywords: | Cytochrome P450 2A13 Single nucleotide polymorphisms Homologous sequences |
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