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三维提取试验检测肠杆菌AmpC酶
引用本文:崔维韵,李奇志,赵群莉,冯玉华. 三维提取试验检测肠杆菌AmpC酶[J]. 中国现代医学杂志, 2003, 13(21): 79-81
作者姓名:崔维韵  李奇志  赵群莉  冯玉华
作者单位:天津铁路中心医院,300140
摘    要:目的 了解临床常见肠杆菌科细菌产AmpCβ-内酰胺酶的产生率。方法 采用头孢西丁筛查试验和三维提取试验对170株茵进行检测。结果 头孢西丁筛查试验阳性29株,三维提取试验阳性5株,可疑4株。结论 头孢西丁筛查试验特异性较差,假阳性高;三维提取法能准确检测AmpCβ-内酰胺酶,其产生率达2.94%。

关 键 词:头孢西丁筛查 三维提取 AmpCβ-内酰胺酶 耐药性 药敏实验
文章编号:1005-8982(2003)21-0079-03
修稿时间:2002-11-29

Detection of AmpC beta-lactamases by cefoxitin three-dimensional extract test
CUI Weiyun,LI Qizhi,ZHAO Qunli,et al.. Detection of AmpC beta-lactamases by cefoxitin three-dimensional extract test[J]. China Journal of Modern Medicine, 2003, 13(21): 79-81
Authors:CUI Weiyun  LI Qizhi  ZHAO Qunli  et al.
Affiliation:CUI Weiyun,LI Qizhi,ZHAO Qunli,et al. The Railway Center Hospital of Tianjin City,Tianjin 300140
Abstract:Objective:To study the occurrence rate of AmpC Beta-Lactmases of clinical bacteria. Methods:To detect 170 isolates by cefoxifin screening test and three-dimensional extract test.Results:29 strains yielded a cefoxifin zone diameter less than 18mm (screen positive), The three-dimensional extract test identified 5 strains producing AmpC Beta-lactamases and 4 strains are suspicious.Conclusion:The cefoxitin screening test has poor specificity ;the three-dimensional extract test may accurately identify the AmpC Beta-Lactamases, its Occurrence rate is 2.94%.
Keywords:AmpC Beta-Lactmnases  Antibiotics Resistance  Drug Sensitive Test
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