| 肿瘤特异性γ δ T细胞扩增体系的建立及其抗肿瘤活性研究 |
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| 引用本文： | 邹畅,赵盼,周文斌,付凡,韩香华,郭吉楠.肿瘤特异性γ δ T细胞扩增体系的建立及其抗肿瘤活性研究[J].现代肿瘤医学,2017(10):1540-1543. |
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| 作者姓名： | 邹畅 赵盼 周文斌 付凡 韩香华 郭吉楠 |
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| 作者单位： | 1. 深圳市人民医院临床医学研究中心,广东 深圳,518020;2. 深圳市合一康生物科技股份有限公司,广东 深圳,518030;3. 深圳市人民医院甲乳外科,广东 深圳,518020;4. 深圳市人民医院泌尿外科,广东 深圳,518020 |
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| 基金项目： | 国家自然科学基金(81402370)，广东省自然科学基金(2015A030313829)，广东省科技项目产学研合作项目(2015B090904007) |
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| 摘 要： | 目的:建立一种体外诱导肿瘤细胞特异性γ δ T细胞的培养体系.方法:从人外周血中分离单个核细胞(PBMCs),用不同浓度的磷酸抗原(IPP)与rhIL-2联合刺激γ δ T细胞增殖,观察并收集细胞,采用流式细胞仪检测刺激后 CD3+Vγ9+的 γ δ T 细胞数量以及比例,并计算其扩增效率.结果:经15天扩增培养后,高浓度组10μg/ml 的IPP联合500IU/ml rhIL-2刺激得到的γ δ T细胞的扩增效率最高,且扩增培养至第9天的γ δ T细胞对肿瘤细胞有一定的杀伤活性.结论:适宜浓度磷酸抗原(IPP)联合rhIL-2能有效体外诱导具有较强抗肿瘤活性γ δ T细胞的扩增.
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| 关 键 词： | 肿瘤 免疫治疗 γδT细胞 |
An exploratory study on the amplification of tumor cell specific γ δ T cells and its anti-tumor activities |
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| Zou Chang,Zhao Pan,Zhou Wenbin,Fu Fan,Han Xianghua,Guo Ji'nan.An exploratory study on the amplification of tumor cell specific γ δ T cells and its anti-tumor activities[J].Journal of Modern Oncology,2017(10):1540-1543. |
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| Authors: | Zou Chang Zhao Pan Zhou Wenbin Fu Fan Han Xianghua Guo Ji'nan |
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| Abstract: | Objective:To amply tumor cells specific peripheral blood γδ T from tumor patients.Methods:Isolating peripheral monocytes (PBMCs) from peripheral blood and amplifying γδ T cells by using IPP plus rhIL-2.Analyzing proportion and total cell amount of CD3+Vγ9+ T cells in total PBMCs by using flow cytometry approach.Exploring the tumor cells killing activities of activated CD3+Vγ9+ T cells by analyzing the target cells viability using Cell Counting Kit-8.Results:After 15 days culturing,the group cultured with the medium supplemented with 10μg/ml IPP plus 500IU/ml rhIL-2 showed highest amplifying efficacy.γδ T cells cultured in the 9th day showed optimal tumor cells killing activities.γδ T activated with cell lysates from tumor cells DU145 showed specific cell killing activities.Conclusion:Tumor cells targeting γδ T could be induced and amplified with optimized IPP plus rhIL-2 formula,which could be a potential tool for immune cell therapy. |
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| Keywords: | tumor immunotherapy γ δ T cells |
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