脂蛋白相关磷脂酶A2基因R92H多态性与汉族人群冠心病易感性的关系

目的探讨脂蛋白相关磷脂酶A2基因R92H多态性与冠心病遗传易感性及冠状动脉病变程度的关系。方法采用单荧光标记探针技术检测261例冠心病患者及263例正常人脂蛋白相关磷脂酶A2基因R92H多态性。结果冠心病组RH基因型和H等位基因频率显著高于对照组(P<0.05和<0.01)。RH基因型、H等位基因人群冠心病风险增高(P均<0.01)。Logistic回归分析显示,R92H多态性是冠心病的独立危险因素(P<0.05)。冠心病组中RH和HH基因型者总胆固醇和低密度脂蛋白胆固醇水平高于RR基因型者(P<0.05和<0.01),高密度脂蛋白胆固醇水平显著低于RR基因型者(P<0.05)。冠心病组中,RH和HH基因型者多支血管病变率比RR基因型者明显升高(P<0.05)。结论脂蛋白相关磷脂酶A2基因R92H多态性与中国汉族人群冠心病相关,携带RH、HH基因型人群发生冠心病风险较高,且与更严重的冠状动脉病变有关。

Association Between Lipoprotein-Associated Phospholipase A2 Gene R92H Polymorphism and Coronary Heart Disease in Han Chinese

Aim To investigate the association between lipoprotein-associated phospholipase A2(Lp-PLA2) R92H single nucleotide polymorphisms (SNP) and susceptibility to coronary heart disease (CHD) and the degree of coronary artery stenosis. Methods 261 CHD patients and 263 normal controls were genotyped by using single-labeled probe technique. Results The frequencies of the RH genotypes and H allele were significantly higher in CHD patients than normal controls (P<0.05 and P<0.01). Compared with RR genotype, the relative risk for CHD in people with RH genotypes was 1.77 (P<0.01). The relative risk for CHD in people with H allele was 1.619 higher than R allele (P<0.01). Binamy logistic regression analysis showed that polymorphism of R92H was an independent risk factor for CHD. There was a significant increase of total cholesterol (TC) and low density lipoprotein cholesterol (LDLC) in the RH HH genotype of CHD patients, while high density lipoprotein cholesterol (HDLC) was lower (all P<0.05). The ratio of multiple vessel diseases was significantly increased in the RH HH genotype of CHD patients (P<0.05). Conclusions The polymorphism of R92H of Lp-PLA2 gene could be associated with risk of CHD in Han Chinese, persons with RH or HH genotypes take higher risk of suffering from CHD.