急性心肌梗死患者血浆外泌体mRNA表达谱分析

目的 比较分析急性心肌梗死(AMI)患者和非心源性胸痛(NCCP)患者血浆外泌体中mRNA的表达差异,并探讨差异表达mRNA对AMI发生发展可能的影响。方法 入选北京朝阳医院和内蒙古包头市中心医院NCCP患者和AMI患者各15例。提取入选者血浆外泌体及外泌体总RNA,高通量测序技术检测AMI患者和NCCP患者血浆中外泌体信使RNA(mRNA)的表达谱,筛选出AMI患者中差异表达的mRNA。对差异表达mRNA进行基因本体(GO)分析和京都基因与基因组百科全书(KEGG)信号通路分析。结果 提取的外泌体为粒径在30~200 nm之间的双层膜小囊泡,表达外泌体标志分子分化簇63(CD63)和热休克蛋白70(HSP70),而不表达甘油醛-3-磷酸脱氢酶(GAPDH),符合外泌体的特征。对入选AMI患者和NCCP患者血浆外泌体中的mRNA表达量进行检测,AMI患者血浆中外泌体转录本数量为64 258条,NCCP患者血浆中外泌体转录本数量为64 374条。其中差异表达的mRNA共1 800条(上调951条,下调849条)。GO和KEGG分析表明上述差异表达的mRNA参与了AMI的生物学调节功能和通路。结论 AMI患者血浆外泌体中mRNA的表达水平与NCCP组比较存在明显的差异,这些差异表达的外泌体mRNA可能在AMI的发生发展过程中发挥重要的作用。

Analysis of the expression profile of plasma exosome mRNAs in patients with acute myocardial infarction

Aim To investigate the expression pattern of plasma exosome mRNA inAMI(acute myocardial infarction ) patients using NCCP(noncardiac chest pain)patients as control group, and determine the possible effects of differentially expressed mRNAs in AMI patitents. Methods Fifteen NCCP andAMI patients were enrolled from Beijing Chao-yang Hospital and Baotou Central Hospital. Exosomes and exosome RNA were extracted from the plasma of selected patients. High-throughput sequencing technology was used to detect the expression of mRNA(message RNA )profile in exosomes in the plasma of patients with AMI and NCCP. GO (gene ontology) and KEGG (kyoto encyclopedia of genes and genomes) were used to analyze differentially expressed mRNAs. Results The size of exosomes was between 30-200 nm. These exosomes express CD63(cluster of differentiation 63) and HSP70 (heat shock proteins 70), but not GAPDH (glyceraldehyde-3-phosphate dehydrogenase), conforming with the characteristics of exosomes. 64258 mRNAs were detected in the plasma exosomes of AMI patients and 64374 mRNAs were detected in the plasma exosomes of NCCP patients.Among these detected mRNAs, 1800 mRNAs were differentially expressed in AMI group (951 mRNAs were upregulated, and 849 mRNAs were down regulated). GO and KEGG analysis showed that these differentially expressed mRNA participated in the biological regulatory functions and pathways of AMI. Conclusion The mRNA expression pattern of plasma exosomes in patients with AMI was significantly different from that in the NCCP group. These differentially expressed exosome mRNAs may play important roles in the occurrence and development of AMI.