| 辽河口芦苇湿地土壤氨氧化菌的时空变化 |
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| 引用本文： | 白洁,董晓,赵阳国.辽河口芦苇湿地土壤氨氧化菌的时空变化[J].中国环境科学,2011,31(11):1870-1874. |
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| 作者姓名： | 白洁 董晓 赵阳国 |
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| 作者单位： | 1. 中国海洋大学环境科学与工程学院,山东青岛266100;海洋环境与生态教育部重点实验室,山东青岛266100
2. 中国海洋大学环境科学与工程学院,山东青岛,266100 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项 |
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| 摘 要： | 采用传统的最大或然数法(MPN)和不依赖于培养的变性梯度凝胶电泳技术(DGGE),对辽河口芦苇湿地土壤中AOB的丰度、多样性进行了解析,并试图探究AOB同土壤可溶性盐分等环境因素之间的关系.结果表明:芦苇湿地土壤中AOB多样性指数时空分布存在较大差异,同一月份中不同站点之间群落结构相似性较大,AOB细菌数量与土壤可溶性盐分之间存在显著负相关关系(P<0.05),与土壤总氮和有机质含量之间存在极显著性正相关关系(P<0.01),表明盐分、总氮和有机质含量是辽河口湿地AOB数量时空分布的重要影响因素.
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| 关 键 词： | 辽河口湿地 氨氧化菌(AOB) 可溶性盐分 有机质 总氮 |
| 收稿时间： | 2011-01-30; |
Characteristics of temporal and spatial distribution of ammonia-oxidizing bacteria in Liaohe Estuary wetland soil |
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| BAI Jie,DONG Xiao,ZHAO Yang-Guo.Characteristics of temporal and spatial distribution of ammonia-oxidizing bacteria in Liaohe Estuary wetland soil[J].China Environmental Science,2011,31(11):1870-1874. |
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| Authors: | BAI Jie DONG Xiao ZHAO Yang-Guo |
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| Affiliation: | BAI Jie1,2,DONG Xiao1,ZHAO Yang-Guo1,2(1.College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China,2.Key Laboratory of Marine Environmental Science and Ecology,Ministry of Education,China) |
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| Abstract: | The nitrification from ammonia to nitrate is a rate-limiting step mediated by ammonia-oxidizing bacteria(AOB).The abundances and diversity of AOB in the soil of reed wetland of Liaohe Estuary were investigated by the most probable number(MPN) method and the denaturing gradient gel electrophoresis(DGGE) technique,and the relationship between AOB abundances and environmental factors,such as soluble salt,was further explored.The results indicated that the temporal and spatial distribution of the AOB diversity ... |
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| Keywords: | Liaohe Estuary wetland ammonia-oxidizing bacteria(AOB) soluble salt organic matter total nitrogen |
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