| 用白细胞粘附抑制法鉴定HBV—TF特异免疫活性 |
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| 引用本文： | 徐燕萍,胡银英,等.用白细胞粘附抑制法鉴定HBV—TF特异免疫活性[J].江西医学院学报,1999,39(4):1-4. |
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| 作者姓名： | 徐燕萍 胡银英 |
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| 作者单位： | 江西省医学科学研究所检测中心!南昌330006(徐燕萍，胡银英，胡昕)，江西省医学科学研究所!南昌330006(陈章林，邹伟民，朱亮) |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 95 60 0 77) |
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| 摘 要： | 选用白细胞粘附抑制试验来鉴定乙肝特异性转移因子(HBV-TF)免疫活性。实验结果表明:经HBV-TF致敏的淋巴细胞与乙肝抗原(HBV-Ag)孵育后,显示出明显的粘附抑制活性,而非特异性转移因子(TFn)致敏淋巴细胞与乙肝抗原孵育、HBV-TF致敏淋巴细胞与甲肝抗原(HAV-Ag)孵育或HBV-TF致敏淋巴细胞与1640液(代替抗原液)孵育时均不能表现出这种抑制作用,说明HBV-TF具有抗原特异性免疫活性。实验证明,本法是体外鉴定HBV-TF免疫活性简便易行的方法。
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| 关 键 词： | 转移因子 乙肝抗原 白细胞粘附抑制试验 猪 |
Determination of Antigen specific Immunologic Activity of Hapatictis Virus B Transfer Factor by Leucocyte Adnerence Inhibition Assay |
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| Xu Yanping Hu Yinying,Chen Zhanglin,Zou Weimin,Zhu Liang,Hu Xin.Determination of Antigen specific Immunologic Activity of Hapatictis Virus B Transfer Factor by Leucocyte Adnerence Inhibition Assay[J].Acta Academiae Medicinae Jiangxi,1999,39(4):1-4. |
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| Authors: | Xu Yanping Hu Yinying Chen Zhanglin Zou Weimin Zhu Liang Hu Xin |
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| Affiliation: | Xu Yanping Hu Yinying * Chen Zhanglin * Zou Weimin * Zhu Liang * Hu Xin |
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| Abstract: | Leucocyte adherence inhibition assay(LAI) was applied to identify antigen specific immunologic activity of hepatitis virus B transfer factor (HBV TF) in vitro.The results indicated that HBV TF could obviously inhibit the adherence of lymphocyte when lymphocyte sensitized by HBV TF were incubated with hepatitis B antigen (HBV Ag),but no such adherence inhibition was found when non specitic transfer factor (TFn), sensitized lumphocytes were incubated with HBV antigen and lymphocytes sensitized by HBV TF incubated with hepatitis A antigen (HBV Ag) or HBV TF sensitized cell and 1 640 (instead of antigen).The results suggest that HBV TF has antigen specific immunological activity.This method is a simple,easy method for identification of immunoactivity of HBV TF in vitro. |
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| Keywords: | transfer factor hepatitis B antigen leukocyte adherence inhibition test swine |
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